Experimental neuroanatomy : a practical approach
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Bibliographic Information
Experimental neuroanatomy : a practical approach
（The practical approach series）
IRL Press at Oxford University Press, c1992
- : hbk
- : pbk
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Includes bibliographical references and index
Description and Table of Contents
- Volume
-
: pbk ISBN 9780199633258
Description
A dramatic increase in the number of practical techniques available to the experimental neuroanatomist have led to major advances in the knowledge and understanding of the nervous system. This book is a laboratory manual for experimental neuroscientists, with an emphasis on ultrastructural analysis. It contains detailed protocols for the preparation of specimens, perfusion-fixation, and the preparation of sections for light and electron microscopy. It covers neuroanatomical techniques including procedures for tract-tracing and chemical characterization of neurones and their processes. These procedures, either alone or in combination, are being used to elucidate complex neuronal interactions. This text should be useful to neuroanatomists and other neuroscientists who wish to apply these experimental techniques.
Table of Contents
- J.P. Bolam: Preparation of central nervous system tissue for light and electron microscopy
- I.J. Llewellyn-Smith, P. Pilowsky, & J.B. Minson: Retrograde tracers for light and electron microscopy
- Y. Smith: Anterograde tracing with PHA-L and biocytin at the electron microscopic level
- P.L. Strick & J.P. Card: Transneuronal mapping of neural circuits with alpha herpesviruses
- S. Totterdell, C.A. Ingham, & J.P. Bolam: Immunocytochemistry I: pre-embedding staining
- C.A. Ingham: Immunocytochemistry II: post-embedding staining
- S.R. Vincent: Histochemistry of endogenous enzymes
- C.R. Gerfen, W.S. Young, III, & P. Emson: In situ hybridization histochemistry with oligonucleotides for localization of messenger RNA in neurones
- E.H. Buhl: Intracellular Lucifer Yellow injection in fixed brain slices
- D.J. Maxwell: Intracellular staining in vivo
- Y. Smith, & J.P. Bolam: Combined approaches to experimental
- neuroanatomy: combined tracing and immunocytochemical techniques for the study of neuronal microcircuits
- Appendix
- Index.
- Volume
-
: hbk ISBN 9780199633265
Description
A dramatic increase in the number of practical techniques available to the experimental neuroanatomist have led to major advances in the knowledge and understanding of the nervous system. This book is a laboratory manual for experimental neuroscientists, with an emphasis on ultrastructural analysis. It contains detailed protocols for the preparation of specimens, perfusion-fixation, and the preparation of sections for light and electron microscopy. It covers neuroanatomical techniques including procedures for tract-tracing and chemical characterization of neurones and their processes. These procedures, either alone or in combination, are being used to elucidate complex neuronal interactions. This text should be useful to neuroanatomists and other neuroscientists who wish to apply these experimental techniques.
Table of Contents
- J.P. Bolam: Preparation of central nervous system tissue for light and electron microscopy
- I.J. Llewellyn-Smith, P. Pilowsky, & J.B. Minson: Retrograde tracers for light and electron microscopy
- Y. Smith: Anterograde tracing with PHA-L and biocytin at the electron microscopic level
- P.L. Strick & J.P. Card: Transneuronal mapping of neural circuits with alpha herpesviruses
- S. Totterdell, C.A. Ingham, & J.P. Bolam: Immunocytochemistry I: pre-embedding staining
- C.A. Ingham: Immunocytochemistry II: post-embedding staining
- S.R. Vincent: Histochemistry of endogenous enzymes
- C.R. Gerfen, W.S. Young, III, & P. Emson: In situ hybridization histochemistry with oligonucleotides for localization of messenger RNA in neurones
- E.H. Buhl: Intracellular Lucifer Yellow injection in fixed brain slices
- D.J. Maxwell: Intracellular staining in vivo
- Y. Smith, & J.P. Bolam: Combined approaches to experimental
- neuroanatomy: combined tracing and immunocytochemical techniques for the study of neuronal microcircuits
- Appendix
- Index.
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