Bioluminescence analysis
著者
書誌事項
Bioluminescence analysis
VCH Verlagsgesellschaft, 1991
- : Weinheim
- : New York
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Today small quantities of light can be measured accurately at low cost. This book outlines the relevant chemical processes behind the luminescence reactions and describes the special kinetic conditions which have to be taken into account when designing analyses of nucleotides, enzymes and substrates. It also focuses on practical aspects, such as how to prepare specimens and reagents, and to record the emission in order to reach a satisfactory analytical performance. All aspects of an analysis, from selection and preparation of specimens to processing of the luminescence data, are covered in detail.
目次
- The phenomenon of chemiluminescence
- principles of analytical applications of luminescence
- enzyme kinetics
- coupling of reactions
- experimental design
- preparation of samples
- generation and measurement of light signals
- fields of application
- samples of application.
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