Laboratory methods in vesicular and vectorial transport
著者
書誌事項
Laboratory methods in vesicular and vectorial transport
(Methods in cell biology / edited by David M. Prescott)
Academic Press, c1991
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book presents proven approaches for studying the secretory and endocytic paths in animal cells. The range of methods covers initial events of translocation into the rough endoplasmic reticulum through to events of vesicular traffic at the cell surface. The chapters have been selected from three recent "Methods in Cell Biology" volumes as those with broad appeal for an expanded audience of students and researchers. Features and benefits include a convenient bench-top format; vectorial transport in the rough endoplasmic reticulum; accessing the cytoplasm; analysis of vesicle fusion; and electron microscopic immunocytochemistry.
目次
- Vectorial transport to the cisternal space of the rough endoplasmic reticulum
- gaining access to the cytoplasm
- cell-free analysis of vesicle fusion
- electron microscopic immunocytochemistry
- analysis of glycoprotein structure
- cell systems for analysis of vesicular traffic
- production and use of anti-idiotypic antibodies.
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