Samples and reagents
著者
書誌事項
Samples and reagents
（Methods of immunological analysis / editor-in-chief, René F. Masseyeff ; editors, Winfried H. Albert, and Norman A. Staines, v. 2）
VCH Verlagsgesellschaft , VCH Publishers, c1993
- : Weinheim
- : New York
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This second volume in a set of 12 on immunological analysis presents samples and reagents of the subject which are required before an understanding of its various applications can be grasped. Aimed at biochemists, immunologists, molecular biologists and pharmacologists, this book provides a complete up-to-date compilation of all methods of immunological analysis, presented in a standard format to allow optimal access to the information. The first three volumes in the series supply a practice-oriented introduction to immunological analysis. The remaining eight volumes provide laboratory sheets of immunoanalytical techniques in various fields of the life sciences, chemistry, and medicine. One or more reliable methods are described for each analyte or class of analytes, typifying alternative approaches. In this way, users will find specific methods for widely-measured analytes that can be implemented without modification. Moreover, they can extract soundly-based principles and experimental techniques on which to construct immunoassays for new or less familiar analytes.
Topics covered include immunoglobulins, complement components, auto-antibodies, tumour-related antigens, and various hormones and blood proteins. Each chapter is designed to give scientists enough information to reproduce the technique described quickly and accurately.
目次
- Part 1 Soluble speciments and samples: preparation and processing of samples
- cell and tissue disintegration. Part 2 Immunogens: from haptens to immunogens
- proteins and polypeptides (native, synthetic, recombinant)
- carbohydrates
- lipids and glycolipids
- nucleic acids
- viruses
- adjuvants. Part 3 Antibodies: polyclonal versus monoclonal antibodies
- production of polyclonal antibodies
- production of monoclonal antibodies
- modification of antibodies
- antibodies with special properties. Part 4 Chemical coupling methods used in immunochemistry: coupling of haptens to carriers and labels
- coupling of antigens and antibodies to labels. Part 5 Standardization of reagents: antigens
- antibodies
- standards and calibrators
- reagents used for separation assays
- quality requirements, conditions for storage.
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