In vitro biological systems
著者
書誌事項
In vitro biological systems
(Methods in toxicology / series editors, Charles A. Tyson, Hanspeter Witschi, v. 1,
Academic Press, c1993
- : pbk
大学図書館所蔵 全11件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
内容説明・目次
- 巻冊次
-
ISBN 9780124612013
内容説明
There is a pressing need among researchers involved in toxicologic investigation for a series of publications that organizes and presents information on the latest experimental methodologies. To address the needs of researchers in toxicology, toxicologic pathology, pharmacology and clinical biochemistry, this new series "Methods in Toxicology" provides comprehensive descriptions of state-of-the-art methods for evaluating drug and chemical toxicity. Thematic volumes focus on mechanistic approaches to the study of toxicity both "in vitro" and "in vivo", taking advantage of the recent advances in the biological and chemical sciences that allow closer scrutiny of the mechanisms by which chemical agents cause organismic or cellular damage. Each volume begins with an introductory chapter that offers a broad guide to the application of methods addressed in that volume. Subsequent chapters contain detailed descriptions of research protocols, accessible both to experts and those new to toxicologic investigation.
Included in each chapter are clearly defined procedures, discussions of limitations of the method, comparative considerations (species, sex, strain), interpretations of results, and explanations of how the methods may serve as alternatives to "in vivo" testing. Volume 1 examines "in vitro" biological systems. "In vitro" methods have provided a valuable tool for toxicological research over the years. Historically, these methods have made significant contributions to our understanding of mechanisms of action toxins and xenobiotic metabolism and, in this context, have provided an indispensable resource for investigative toxicology. More recently, the value of "in vitro" systems as toxicity tests for chemical safety and hazard evaluation has been recognized and explored. Whether the "in vitro" approach is employed as a model system for investigative research or as a toxicity test for risk assessment, a critical component is the biological system. Selection and successful culturing of the appropriate cell, tissue or organ for a particular scientific purpose is essential for a satisfactory outcome.
The objective of this volume is to provide both beginning, as well as established, researchers with basic techniques employed by widely recognized scientists to prepare and maintain the biological components of "in vitro" model systems. The compilation is not intended to be exhaustive but to provide a set of pivotal methods of value to researchers in the field of toxicology. The methods have been organized by organ systems for easy reference. Although "in vitro" studies in isolated organelles are important in toxicological research, these methods have not been included in this volume.
目次
- Part 1 Neural and neuromuscular systems: hippocampal slices, P.G. Aitken
- organotypic neural cultures, F.J. Seil
- reaggregate cells for neurotoxicological studies, A. Heller, et al
- cytotoxicity in murine neocortical cell culture, K. Rose, et al
- cultures of adult trigeminal ganglion neurons, T.K. Baumann
- adrenomedullary chromaffin cells - a well-characterized model system for toxicological studies, J. Knoth-Anderson and M.B. Abou-Donia
- PC 12 cells, G.E. Isom and J.L. Barowitz. Part 2 Ocular system: corneal epithelial and endothelial cell cultures, M.M. Jumblatt
- isolation and culture of retinal epithelium, P. Gouras. Part 3 Respiratory system: isolation and culture of type II alveolar epithelial cells, J.N. Finkelstein
- isolation of nonciliated bronchiolar (Clara) epithelial cells from mouse lung, A.M. Malkinson, et al
- rat tracheal epithelial cell cultures for studies in toxicology and carcinogenicity, T.E. Gray, et al. Part 4 Cardiovascular system: preparation of primary cultures of postnatal rat myocardial cells for toxicological studies, A.A. Welder and D. Acosta
- aortic endothelial and smooth muscle cell cultures, K.S. Ramos and L.R. Cox
- vessel cylinders, R.K. Hester and K.S. Ramos. Part 5 Gastro-intestinal system: gastric mucosal cell culture for toxicological study, H. Hiraishi, et al
- small intestinal enterocytes, T.Y. Aw, et al
- maintenance and characterization of an organ-culture system for colonic mucosa from the rat, K.J. Finney. Part 6 Liver slices: precision-cut rat liver slices in dynamic organ culture for structure-toxicity studies, K. Brendel, et al. Part 7 Hepatocytes: isolation of hepatocytes by collagenase perfusion, P.O. Seglen
- preparation of primary monolayer cultures of postnatal rat liver cells for hepatotoxic assessment of xenobiotics, J.C. Davila and D. Acosta
- isolation and culture of hepatocytes from different laboratory species, C.A. McQueen
- isolation of human hepatocytes by biopsy perfusion methods, K.L. Allen and C.E. Green
- human hepatocyte cultures, C. Guguen-Guillouzo and A. Guillouzo. Part 8 Liver - other cell systems: hepatocyte and spleen cell systems, N.E. Kaminski, et al
- isolation and culture of hepatic non-parenchymal cells, S.L. Friedman
- proliferating lines of rat liver epithelial cells, G.M. Williams and A.M. Bennett
- isolation and culture of liver epithelial cells from carcinogen-treated rats, N.L. Thompson and N. Fausto. Part 9 Kidney - proximal tubule fragments: proximal renal tubule preparations isolated from rat or rabbit kidneys without the use of collagenase, K. Brendel, et al
- suspension culture of rabbit renal proximal tubules, K.G. Dickman and L. Mandel
- perfusion method for isolation of renal proximal tubules from several species, J.E. Dabbs and C.E. Green
- isolation and maintenance of milligram quantities of rabbit renal proximal straight and convoluted tubules, C.E. Ruegg and L.J. Mandel. (Part contents).
- 巻冊次
-
: pbk ISBN 9780124612020
内容説明
There is a pressing need among researchers involved in toxicologic investigation for a series of publications that organizes and presents information on the latest experimental methodologies. To address the needs of researchers in toxicology, toxicologic pathology, pharmacology, and clinical biochemistry, this new serial - "Methods in Toxicology" - provides comprehensive descriptions of state-of-the-art methods for evaluating drug and chemical toxicity. Thematic volumes focus on mechanistic approaches to the study of toxicity both "in vitro" and "in vivo", taking advantage of the recent advances in the biological and chemical sciences that allow closer scrutiny of the mechanisms by which chemical agents cause organismic or cellular damage. Each volume begins with an introductory chapter that offers a broad guide to the application of methods addressed in that volume. Subsequent chapters contain detailed descriptions of research protocols, accessible both to experts and those new to toxicologic investigation.
Included in each chapter are clearly defined procedures, discussions of limitations of the method, comparative considerations (species, sex, strain), interpretations of results and explanations of how the methods may serve as alternatives to "in vivo" testing. Each volume of "Methods in Toxicology" is available in case binding for the library and wire-o-binding for the laboratory. "In vitro" methods have provided a valuable tool for toxicological research over the years. Historically, these methods have made significant contributions to our understanding of mechanisms of action toxins and xenobiotic metabolism and, in this context, have provided an indispensable resource for investigative toxicology. More recently, the value of "in vitro" systems as toxicity tests for chemical safety and hazard evaluation has been recognized and explored. Whether the "in vitro" approach is employed as a model system for investigative research or as a toxicity test for risk assessment, a critical component is the biological system. Selection and successful culturing of the appropriate cell, tissue or organ for a particular scientific purpose is essential for a satisfactory outcome.
The objective of this volume is to provide both beginning, as well as established, researchers with basic techniques employed by widely recognized scientists to prepare and maintain the biological components of "in vitro" model systems. The compilation is not intended to be exhaustive but to provide a set of pivotal methods of value to researchers in the field of toxicology. The methods have been organized by organ systems for easy reference. Although "in vitro" studies in isolated organelles are important in toxicological research, these methods have not been included in this volume.
目次
- Part 1 Neural and neuromuscular systems: hippocampal slices, P.G. Aitken
- organotypic neural cultures, F.J. Seil
- reaggregate cells for neurotoxicological studies, A. Heller, et al
- cytotoxicity in murine neocortical cell culture, K. Rose, et al
- cultures of adult trigeminal ganglion neurons, T.K. Baumann
- adrenomedullary chromaffin cells - a well-characterized model system for toxicological studies, J. Knoth-Anderson and M.B. Abou-Donia
- PC 12 cells, G.E. Isom and J.L. Barowitz. Part 2 Ocular system: corneal epithelial and endothelial cell cultures, M.M. Jumblatt
- isolation and culture of retinal epithelium, P. Gouras. Part 3 Respiratory system: isolation and culture of type II alveolar epithelial cells, J.N. Finkelstein
- isolation of nonciliated bronchiolar (Clara) epithelial cells from mouse lung, A.M. Malkinson, et al
- rat tracheal epithelial cell cultures for studies in toxicology and carcinogenicity, T.E. Gray, et al. Part 4 Cardiovascular system: preparation of primary cultures of postnatal rat myocardial cells for toxicological studies, A.A. Welder and D. Acosta
- aortic endothelial and smooth muscle cell cultures, K.S. Ramos and L.R. Cox
- vessel cylinders, R.K. Hester and K.S. Ramos. Part 5 Gastro-intestinal system: gastric mucosal cell culture for toxicological study, H. Hiraishi, et al
- small intestinal enterocytes, T.Y. Aw, et al
- maintenance and characterization of an organ-culture system for colonic mucosa from the rat, K.J. Finney. Part 6 Liver slices: precision-cut rat liver slices in dynamic organ culture for structure-toxicity studies, K. Brendel, et al. Part 7 Hepatocytes: isolation of hepatocytes by collagenase perfusion, P.O. Seglen
- preparation of primary monolayer cultures of postnatal rat liver cells for hepatotoxic assessment of xenobiotics, J.C. Davila and D. Acosta
- isolation and culture of hepatocytes from different laboratory species, C.A. McQueen
- isolation of human hepatocytes by biopsy perfusion methods, K.L. Allen and C.E. Green
- human hepatocyte cultures, C. Guguen-Guillouzo and A. Guillouzo. Part 8 Liver - other cell systems: hepatocyte and spleen cell systems, N.E. Kaminski, et al
- isolation and culture of hepatic non-parenchymal cells, S.L. Friedman
- proliferating lines of rat liver epithelial cells, G.M. Williams and A.M. Bennett
- isolation and culture of liver epithelial cells from carcinogen-treated rats, N.L. Thompson and N. Fausto. Part 9 Kidney - proximal tubule fragments: proximal renal tubule preparations isolated from rat or rabbit kidneys without the use of collagenase, K. Brendel, et al
- suspension culture of rabbit renal proximal tubules, K.G. Dickman and L. Mandel
- perfusion method for isolation of renal proximal tubules from several species, J.E. Dabbs and C.E. Green
- isolation and maintenance of milligram quantities of rabbit renal proximal straight and convoluted tubules, C.E. Ruegg and L.J. Mandel. (Part contents).
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