書誌事項

Haemopoiesis : a practical approach

edited by N.G. Testa and G. Molineux

(The practical approach series)

IRL Press at Oxford University Press, c1993

1st ed

  • :hbk.
  • :pbk.

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注記

Includes bibliographical references

内容説明・目次

巻冊次

:pbk. ISBN 9780199633654

内容説明

The cells and regulators involved in haemopoiesis are well characterized, providing biochemists and cell and molecular biologists with an exciting system in which to study normal and abnormal growth and differentiation. Rapid progress has also been made in the application of research techniques to human cells, giving rise to new therapeutic approaches to diseases such as leukaemia. This is a detailed guide to the most important methods in use in this area today, and will be of major interest to haematologists and researchers in many areas of basic and medical research.

目次

  • 1. In vivo assays for multipotential and marrow repopulating cells
  • 2. Enrichment of progenitor cell populations from murine and human haemopoietic tissues
  • 3. In vitro clonal assays for murine multipotential and lineage restricted myeloid progenitor cells
  • 4. Long-term mouse bone marrow cultures
  • 5. Clonal and long-term cultures using human bone marrow
  • 6. Isolation and culture of factor-dependent haemopoietic cell lines
  • 7. Development of haemopoietic cells in liquid culture
  • 8. Analysis of growth factor binding to haemopoietic cells
  • 9. Signal transduction
  • 10. Retroviral gene transfer into haemopoietic cells
  • 11. Assays of the haemopoietic microenvironment
  • 12. The visualization of haemopoietic and stromal cell types
  • 13. Autologous bone marrow transplantation using cultured marrow cells in patients with leukaemia
  • 14. Analysis of gene expression in haemopoietic cells
  • 15. Molecular analysis of clonality and transplantation in haemopoiesis
巻冊次

:hbk. ISBN 9780199633661

内容説明

A detailed guide to the most important methods in use in the area of experimental haematology, this volume should be of interest to haematologists and researchers in many areas of basic and medical research. The cells and regulators involved in haemopoiesis are well characterized, providing biochemists and cell and molecular biologists with an exciting system in which to study normal and abnormal growth and differentiation. Rapid progress has also been made in the application of research techniques to human cells, giving rise to new therapeutic approaches to diseases such as leukaemia. The book is aimed at postgraduates and researchers in haematology, cell biology, molecular biology and immunology.

目次

  • "In vivo" assays for multipotential and marrow repopulating cells, B.I. Lord
  • enrichment of progenitor cell populations from murine and human haemopoietic tissues, B.I. Lord and J.C.W. Marsh
  • "in vitro" clonal assays for murine multipotential and lineage restricted myeloid progenitor cells, C.M. Heyworth and E. Spooncer
  • long-term mouse bone marrow cultures, E. Spooncer, et al
  • clonal and long-term cultures using human bone marrow, L.H. Coutinho, et al
  • isolation and culture of factor-dependent haemopoietic cell lines, E. Spooncer, et al
  • development of haemopoietic cells in liquid culture, O. Kan, et al
  • analysis of growth factor binding to haemopoietic cells, F. Walker and C.M. Heyworth
  • signal transduction, P.J. Owen, et al
  • retroviral gene transfer into haemopoietic cells, L.J. Fairbairn and E. Spooncer
  • assays of the haemopoietic microenvironment, A.J. Friedenstein, et al
  • the visualization of haemopoietic and stromal cell types, T.D. Allen and E.A. de Winter
  • autologous bone marrow transplantation using cultured marrow cells in patients with leukaemia, L.H. Coutino, et al
  • analysis of gene expression in haemopoietic cells, G.J. Cowling, et al
  • molecular analysis of clonality and transplantation in haemopoiesis, J.C.W. Marsh, et al.

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