Growth factors : a practical approach
著者
書誌事項
Growth factors : a practical approach
（The practical approach series, 119）
IRL Press at Oxford University Press, c1993
- : hbk
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199633593
内容説明
Growth factors play an important role in the regulation of proliferation and differentiation of specific cell types. This is a guide to studying growth factors, from the search for and characterization of new activities, to "in vivo" analysis and molecular biology approaches such as DNA cloning. Although cytokines is included in the list of growth factors, the book avoids protocols relating specifically to cytokines and this volume should be seen as complementary to the volume "Cytokines: A Practical Approach" in the same series.
目次
- 1. Types of growth factor activity: detection and characterization of new growth factor activities
- 2. Detection of polypeptide growth factors: application of specific bio-assays and PCR technology
- 3. Induction of DNA synthesis and cell division in quiescent cells in response to small peptide growth factors
- 4. Novel gene transcription in response to growth factors: gene isolation by differential plaque hybridization
- 5. Embryological assays for growth factors
- 6. The role of TGF- in bone growth and bone repair
- 7. Localization of growth factor mRNA in tissue sections by in-situ hybridization
- 8. Detection and characterization of growth inhibitory factors: epidermal inhibitory pentapeptide (EIP)
- 9. Expression of growth factors in microbial systems
- 10. Immunoassays for growth factors
- 11. Determination of growth factor receptor distribution by immunocytochemistry or autoradiography and quantitation by ligand binding or FACS analysis
- Appendices
- 巻冊次
-
: hbk ISBN 9780199633609
内容説明
Growth factors play an important role in the regulation of proliferation and differentiation of specific cell types. This is a guide to studying growth factors, from the search for and characterization of new activities, to "in vivo" analysis and molecular biology approaches such as DNA cloning. Although cytokines is included in the list of growth factors, the book avoids protocols relating specifically to cytokines and this volume should be seen as complementary to the volume "Cytokines: A Practical Approach" in the same series.
目次
1: I.A. McKay: Types of growth factor activity: detection and characterization of new growth factor activities. 2: E.J.J. Van Zoelen, B. Lelaey, N. Van der Burg, and D. Huylebroeck: Detection of polypeptide growth factors: application of specific bio-assays and PCR technology. 3: A.N. Corps, and K.D. Brown: Induction of DNA synthesis and cell division in quiescent cells in response to small peptide growth factors. 4: P. Sanjeeva Reddy and B.H. Cochran: Novel gene transcription in response to growth factors: gene isolation by differential plague hybridization. 5: J.M.W. Slack: Embryological assays for growth factors. 6: R.T. Ballock and A.B. Roberts: The role of TGF- in bone growth and bone repair. 7: R.J. Akhurst: Localization of growth factor mRNA in tissue sections by in-situ hybridization. 8: P. Robinson, P. Whitehead, and W. Hume: Detection and characterization of growth inhibitory factors: epidermal inhibitory pentapeptide (EIP). 9: T.J. Dudgeon, J.M. Clements, and M.G. Hunter: Expression of growth factors in microbial systems. 10: S.J. Capper: Immunoassays for growth factors. 11: C. Linge and M.R. Green: Determination of growth factor receptor distribution by immunocytochemistry or autoradiography and quantitation by ligand binding or FACS analysis. Appendices
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