Hybridization techniques for electron microscopy
Author(s)
Bibliographic Information
Hybridization techniques for electron microscopy
CRC Press, c1993
Available at 11 libraries
  Aomori
  Iwate
  Miyagi
  Akita
  Yamagata
  Fukushima
  Ibaraki
  Tochigi
  Gunma
  Saitama
  Chiba
  Tokyo
  Kanagawa
  Niigata
  Toyama
  Ishikawa
  Fukui
  Yamanashi
  Nagano
  Gifu
  Shizuoka
  Aichi
  Mie
  Shiga
  Kyoto
  Osaka
  Hyogo
  Nara
  Wakayama
  Tottori
  Shimane
  Okayama
  Hiroshima
  Yamaguchi
  Tokushima
  Kagawa
  Ehime
  Kochi
  Fukuoka
  Saga
  Nagasaki
  Kumamoto
  Oita
  Miyazaki
  Kagoshima
  Okinawa
  Korea
  China
  Thailand
  United Kingdom
  Germany
  Switzerland
  France
  Belgium
  Netherlands
  Sweden
  Norway
  United States of America
Note
Includes bibliographical references and index
Description and Table of Contents
Description
Hybridization Techniques for Electron Microscopy examines the use of in situ hybridization techniques, including an overview of current perspectives and future developments. The book features in situ methods for fluorescence probes and confocal scanning microscopes. Three in situ hybridization methods for electron microscopes are analyzed: the non-embedded tissue method using ultrathin frozen sections, pre-embedded method, and post-embedded method using material embedded in hydrophilic resin. Positive and negative features are discussed, and clear instructions regarding implementation of techniques are provided.
Particular aspects of the techniques are examined in detail, such as preparation of tissue, pretreatment, hybridization procedures, revelation (autoradiography and immunocytology) and checking procedures, in addition to the illustration, interpretation, and discussion of methods and results. The main applications described include virus detection, chromosomal gene mapping, detection of ribosomic nucleic acid, and detection of messenger RNA in animals and plants. Hybridization Techniques for Electron Microscopy is an excellent reference for cytologists, cell biologists, histochemists, cytochemists, molecular endocrinologists, and neuroendocrinologists.
Table of Contents
Introduction (G. Morel). The Molecular Principles of In Situ Hybridization (C. Perrett). Principles of Probe Preparation for In Situ Hybridization (P. Couble). DNA In Situ Hybridization and Confocal Microscopy (H. Van Dekken, G.J. Brakenhoff, and J.G.J. Bauman). In Situ Hybridization on Semi-Thin Sections (C. Pechoux and G. Morel). Pre-Embedding In Situ Hybridization (Alain Trembleau). In Situ Hybridization on Ultrathin Frozen Sections (G. Morel). Ultrastructural In Situ Hybridization of mRNA on Lowicryl Sections Using Quick-Freezing, Freeze-Substitution and Low Temperature Embedding (L. Frappart and D. Le Guellec). Electron Microscopic of rRNA In Situ Hybridization on Lowicryl Sections (J.G. Fournier and F. Escaig-Haye). Procedures of In Situ Nucleic Acid Hybridization to Detect Viral DNA and RNA in Cells by Electron Microscopy (F. Puvion-Dutilleul). Electron Microscopic In Situ Hybridization to RNA or DNA in Plant Cells (J. Brangeon and L. Sossountzov).
by "Nielsen BookData"