Monitoring genetically manipulated microorganisms in the environment
著者
書誌事項
Monitoring genetically manipulated microorganisms in the environment
（Wiley Biotechnology series）
Wiley, c1993
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Monitoring Genetically Manipulated Microorganisms in the Environment Clive Edwards, Department of Genetics and Microbiology, University of Liverpool, UK Genetically-manipulated microorganisms (GEMs) have been proposed for a variety of applications that would involve their release into open environments that already harbour a diverse and often highly active microflora. This proposal is controversial, especially in view of the problems in studying the fate and survival of GEMs in natural ecosystems. This book describes methods for the detection of target organisms in heterogeneous populations. The tendency of some species to adopt viable but non-culturable states is addressed. Molecular genetic methods that have the potential to overcome this problem are presented. These are also illustrated by their application as detection methods in aquatic environments. Terrestrial habitats are discussed using a description of a contained microcosm for release and recovery of genetically modified Gram-positive bacteria.
Detailed methods for the quantitative extraction of microorganisms from soil are reviewed, along with an overview of experimental systems that have attempted to define the fate of GEMs in soil as well as to assess gene transfer events. The final chapter deals with models that describe microbial interactions in the environment. Although the chapters in this book are principally aimed at those working with genetically manipulated microorganisms there is no doubt that much of what is discussed is equally applicable to increasing our understanding of microbial ecology in general.
目次
The Significance of In Situ Activity on the Efficiency of Monitoring Methods (C. Edwards). Genotypic and Phenotypic Methods for the Detection of Specific Released Microorganisms (J. Saunders & V. Saunders). In Situ Detection of Plasmid Transfer in the Aquatic Environment (R. Pickup, et al.). Composting as a Model System for Monitoring the Fate of Genetically Manipulated Gram-Positive Bacteria (W. Amner, et al.). Extraction, Detection and Identification of Genetically Engineered Microorganisms from Soils (A. O'Donnell & D. Hopkins). Gene Transfer in Terrestrial Environments and the Survival of Bacterial Inoculants in Soil (E. Wellington, et al.). Mathematical Modeling of Genetically Engineered Microorganisms in the Environment (J. Prosser). Index.
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