Mitochondrial dysfunction
Author(s)
Bibliographic Information
Mitochondrial dysfunction
（Methods in toxicology / series editors, Charles A. Tyson, Hanspeter Witschi, v. 2）
Academic Press, c1993
- : pbk
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Description and Table of Contents
- Volume
-
ISBN 9780124612051
Description
Methods in Toxicology, Volume 2: Mitochondrial Dysfunction provides a source of methods, techniques, and experimental approaches for studying the role of abnormal mitochondrial function in cell injury. The book discusses the methods for the preparation and basic functional assessment of mitochondria from liver, kidney, muscle, and brain; the methods for assessing mitochondrial dysfunction in vivo and in intact organs; and the structural aspects of mitochondrial dysfunction are addressed. The text also describes chemical detoxification and metabolism as well as specific metabolic reactions that are especially important targets or indicators of damage. The methods for measurement of alterations in fatty acid and phospholipid metabolism and for the analysis and manipulation of oxidative injury and antioxidant systems are also considered. The book further tackles additional methods on mitochondrial energetics and transport processes; approaches for assessing impaired function of mitochondria; and genetic and developmental aspects of mitochondrial disease and toxicology. The text also looks into mitochondrial DNA synthesis, covalent binding to mitochondrial DNA, DNA repair, and mitochondrial dysfunction in the context of developing individuals and cellular differentiation. Microbiologists, toxicologists, biochemists, and molecular pharmacologists will find the book invaluable.
Table of Contents
Contributors
Preface
Introduction: Criteria for Assessing Normal and Abnormal Mitochondrial Function
Section I Systems for Study of Mitochondrial Dysfunction
1. Mitochondrial Isolation from Liver and Kidney: Strategy, Techniques, and Criteria for Purity
2. Mitochondrial Isolation from Brain: Strategy, Techniques, and Criteria for Purity
3. Intact Rat Brain Mitochondria from a Single Animal: Preparation and Properties
4. Study of Skeletal Muscle Mitochondrial Dysfunction
5. Small-Scale Preparation of Skeletal Muscle Mitochondria and Its Application in the Study of Human Disease
6. Metabolic Control Analysis as a Method to Assess Mitochondrial Dysfunction
Section II Assessment of Mitochondrial Dysfunction in Vivo and in Organ Systems
7. Commentary: Methods for in Vivo Assessment of Mitochondrial Function 98
8. Noninvasive Assessment of Mitochondrial Function by Breath Analysis Using Ketoisocaproic Acid
9. Absorption Spectroscopy for Assessment of Mitochondrial Function in Vivo
10. Analysis of Mitochondrial Function by Carbon-13 Nuclear Magnetic Resonance Spectroscopy in Intact Tissues
11. Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy in the Study of Mitochondrial Metabolism
12. Mitochondrial Dysfunction in Ischemic Organs
13. Screening for Mitochondrial Cytopathy: The Subanaerobic Threshold Exercise Test
Section III Assay of Mitochondrial Structural Integrity
14. Morphometry of Mitochondria: Size, Internal Structure, Subcellular Distribution, and Three-Dimensional Reconstruction
15. Megamitochondria
16. Redox Status and Mitochondrial Inner Membrane Permeability
Section IV Mitochondrial Metabolism and Bioactivation and Detoxification Systems
17. Assessment of Mitochondrial Glutathione as a Measure of Cell Injury
18. Experimental Manipulation of Mitochondrial Glutathione Concentrations
19. Determination of Flux, Activity, and Activity State of Mitochondrial I+/--Keto Acid Dehydrogenase Complexes
20. Regulation of Oxygen Uptake in the Liver Lobule by Oxygen Tension
21. Generation of Reactive Oxygen Metabolites and Oxidative Damage in Mitochondria: Role of Calcium
22. Fatty Acid Metabolism and Reye's Syndrome
23. Antioxidative Function of Vitamin I* and Ubiquinols
24. Mitochondrial NADPH
25. Long-Chain Acyl-CoA Metabolism by Mitochondrial Carnitine Palmitoyltransferase: A Cell Model for Pathological Studies
Section V Mitochondrial Energetics and Transport Processes
26. Identification of Mitochondrial Dysfunction at Coupling Site I: Loss of Activity of NADH-Ubiquinone Oxidoreductase during Myocardial Ischemia
27. Identification of Mitochondrial Dysfunction at Coupling Site II
28. Cellular Calcium and Mitochondrial Dysfunction
29. Chronic Alcoholism and the Mitochondrial FoFi-ATP Synthase
30. Mitochondrial Pyrophosphate Metabolism in Health and Disease
31. Toxic Effects of Calcium on Mitochondria
32. Calcium and the Regulation of Intramitochondrial Dehydrogenases
33. Use of Fluorescent Probes to Monitor Mitochondrial Membrane Potential in Isolated Mitochondria, Cell Suspensions, and Cultured Cells
Section VI Genetic and Developmental Approaches to Study of Mitochondrial Dysfunction
34. Commentary: Genetic Approaches to Mitochondrial DNA Diseases of Oxidative Phosphorylation
35. Developmental Aspects of Mitochondrial Dysfunction
36. Mitochondrial DNA Synthesis
37. Covalent Binding of Reactive Intermediates of Xenobiotics to Mitochondrial DNA
38. Mitochondrial DNA Repair and Cell Injury
Index
- Volume
-
: pbk ISBN 9780124612068
Description
Disruption of mitochondrial energy production occurs commonly during cell injury and death. Efforts to understand mechanisms involved in toxic cell injury and to develop protective treatments therefore require a definition of the role of mitochondrial dysfunction. This volume approaches mitochondrial preparation and functional assessment from the perspective of toxic cell injury, with chapters contributed by experts representing a great diversity of biomedical disciplines. It is conveniently divided into six sections, addressing general methods, "in vivo" assessment, structure, detoxication and metabolic targets, bioenergetic and transport functions, and genetic analysis. The variety of approaches and subjects reflects the fundamental importance of mitochondria in aerobic cell survival. This volume should be of interest to researchers and students in toxicology, veterinary toxicology, toxicologic pathology, pharmacology and clinical biochemistry. It should provide a useful contemporary collection of papers critical to the research of all who are investigating mechanisms of cell injury.
Table of Contents
Part 1: Systems for Study of Mitochondrial Dysfunc"don: L.H. Lash and J.M. Sail, NCtochondrial Isolation from Liver and Kidney: S trategy, Techniques, and Criteria for Purity. N-R . Sirm, Nfitochondrial Isolation from Brain: Strategy, Techniques, and Criteria for Purity. C. -P. Lee, M. Sciamanna, and P.L. Peterson, Intact Rat Brain Nfitochondria from a Single Animal: Preparation and Properties. M. Birch-Machin, S. Jackson, R.S. Kler, and D.M. Turnbull, Study of Skeletal Muscle Mtochondrial Dysfifnction. C. -P. Lee, M.E. Martens, and S.H. Tsang, Small-Scale Preparation of Skeletal Muscle NEtochondria and Its Application in the Study of Human Disease. H.V Westerhoff, B.H, Groen, and C. Van den Bogert, Applications of Metabolic Control Theory to Assessment of Mitochondrial Dysfunction. Part 2: Assessment of Mitochondrial Dysfunction in vivo and in Organ Systems: B. Chance, Commentary: Methods for in Vivo Assessment of Nfitochondrial Function. B.H. Lauterburg, Noninvasive Assess. ment of Nfitochondrial Function by Breath Analysis Using Ketoisocaproic Acid. CA. Piantadosi, Absorption Spectroscopy for in Vivo Assessment of Mitochondrial Dysfunction. C.R. MalloyA.D. Sherry, and FM. Jeffery, Analysis of Mtochondrial Function by 13C-NMR in Intact Tissues. M.R. Laughlin , 3 IP-NMR in the Study of Mitochondrial Metabolism. T Inoue andk. Tagawa, Mitochondrial Dysfunction in Ischemic Organs. R.J.M. Lane, Screening for Mitochondrial Cytopathy: The Subanaerobic Threshold Exercise Test. Part 3: Assay of Mitochondrial Structural Integrity: -S.R. Kayar, Morphometry of Mitochondria: Size,Internal Structure,Subcellular Distribution,and Three-Dimensional Reconstruction. C.L. Hoppel and B. Tandler, Megarnitochondria. K. Li-QUOc and D. Li-Qudc, Redox Statu4 and Mitochondrial Inner Membrane Permeability. Part 4: Mitochondrial Metabolism and Bioacdvatio@n and Detoxiflcation Systems: DJ. Reed, Assessment of Nfitochondrial Glutathione as a Measure of Cell Injury. X. Shan, M. Finkelstein, D.P. Jones, and M.W. Anders, Experimental Manipulation of NfitochondrialGlutathioneConcen@ons.RA. Harris, Y Zhao, and R. Paxton, Determination of Flux, Activity, and Activity State of Nfitochondrial a-Ketoacid Dehydrogenase Complexes. R.G. Thurman, Y Nakagawa, T Matsumara, JJ. Lemasters, and FC. Ka@n, Regulation of Oxygen Uptake in the Liver Lobule by Oxygen Tension. A.E. Vercesi and M.E. Ho@, Generation of Reactive Oxygen Metabolites and Oxidative Damage in Nttochondria: Role of Calcium. C.R. Roe, D.S. Roe, and D.S. Millington, Fatty Acid Metabolism and Reyes Syndrome. V Kagan and L. Packer, Antioxidative Function of Vitamin E and Ubiquinols. R.G. Thurman and FC. Kauffmn, NEtochondrial NADPH. J.B. McMillan, E.K. Hudson, and LM. Buja, Long-chain Acyl-CoA Metabolism by Mitochondrial Carnitine Palmitoyltranferase: A Cell Model for Pathological Studies. Part 5: Mitrochondrial Energetics and Transport Processes: W. Rouslin, Identification of Mitochondrial Dysfunction at Coupling Site I:
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