Capillary electrophoresis technology
著者
書誌事項
Capillary electrophoresis technology
（Chromatographic science series, v. 64）
Dekker, c1993
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Tracing the sequence of observations that has led to current understanding in the field, this reference presents the basic concepts, instrumentation and applications of capillary electrophoresis, examining its many features such as high-power resolution, high-mass sensitivity, overall sensitivity and low-sample volume requirements. This work highlights the use of capillary elctrophoresis for the identification, separation, detection and characterization of substances on the molecular counting level. Illustrating the major technical maneuvers for common operations and applications, Capillary Electrophoresis Technology outlines the theoretical concepts and mathematical expressions of capillary electrophoresis; describes advances in instrumentation hardware and detection systems. It explains the advantages and limitations of the different variants of capillary electrophoresis; and provides extra coverage of areas in which capillary electrophoresis has grown increasingly popular, including the identification and characterization of small molecules and macromolecules. Written by experts in the field, this book is aimed at analytical and clinical chemists and biochemists, chemical engineers, biologists, pharmacists, biotechnologists and students in these disciplines.
目次
- Overview - capillary electrophoresis - introduction and assessment, Barry L. Karger and Frantisek Foret, micellar electrokinetic chromatography, Shigeru Terabe, conventional isoelectric focusing and immobilized pH gradients - an overview, Pier Giorgio Righetti and Marcella Chiari
- buffer system - the buffer in capillary zone electrophoresis, George M. Janini and Haleem J. Issaq, organic solvents in capillary electrophoresis, Ernst Kenndler, controlling migration behavior in capillary electrophoresis - optimization strategies for method development, Morteza G. Khaledi et al, dynamic changes of electrolyte systems in zone electrophoresis, Petr Bocek and Petr Gebauer
- capillary column - chemical derivatization of fused silica capillaries, Fred E. Regnier and Dan Wu, technology of separation capillaries for capillary zone electrophoresis and capillary gel electrophoresis - the chemistry of surface modification and formation of gels, Gerhard Schomburg, capillary electrophoresis with coated capillaries, Jorg Kohr and Heinz Engelhardt, capillary zone electrophoresis of biopolymers with hydrophilic fused-silica capillaries, Ziad El Rassi and Wassim Nashabeh, covalent surface modification for capillary electrophoresis - characterization and effect of non-ionic bondings on separations in capillary electrophoresis, Ann M. Dougherty and Mark R. Schure
- instrumentation - direct control of electroosmotic flow in capillary electrophoresis by using an external electric field, Pei Tsai and Cheng S. Lee, semipreparative capillary electrophoresis and its advantages, Takao Tsuda, micropreparative capillary electrophoresis, Chuzo Fujimoto and Kiyokatsu Jinno, mass spectrometric detection for capillary electrophoresis, Richard D. Smith and Harold R. Udseth, capillary zone electrophoresis-mass spectrometry - continuous flow fast-atom bombardment and electrospray ionization, Kenneth B. Tomer, optical detection schemes for capillary electrophoresis, Edward S. Yeung, laser-induced fluorescence detection for capillary electrophoresis - a powerful analytical tool for the separation and detection of trace amounts of analytes, Luis Hernandez et al
- applications - chiral separation by capillary electrophoresis and electrokinetic chromatography, Koji Otsuka and Shigeru Terabe, the sheath-flow cuvette in DNA sequencing by capillary gel electrophoresis and two-spectral-channel, laser-induced fluorescence detection, Jianzhong Zhang et al, the use of capillary electrophoresis in clinical diagnosis, Norberto A. Guzman et al, the utility of capillary electrophoresis in forensic science, David M. Northrop, capillary electrophoresis and electrokinetic capillary chromatography of drugs in body fluids, Wolfgang Thormann, quantitative analysis with capillary zone electrophoresis, Arthur M. Hoyt, capillary polyacrylamide gel electrophoresis, Andras Guttman, use of cyclodextrins in capillary electrophoresis, Fanali. (Part contents). .
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