NMR of macromolecules : a practical approach
著者
書誌事項
NMR of macromolecules : a practical approach
（The practical approach series, 134）
IRL Press at Oxford University Press, c1993
- : hbk
- : pbk
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注記
Includes bibliographical references
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199632244
内容説明
The last 10-15 years have seen an enormous upsurge in the use of nuclear magnetic resonance (NMR) to study the conformation and interactions of biological macromolecules. NMR is now second in importance only to x-ray crystallography in structural molecular biology. As a result, more and more biologists are seeking to use NMR to solve their structural problems. There are several very good books which give an introduction to the theory of modern NMR spectroscopy, but
much less is available on the practical side which is specifically tailored to the special technique required.
The aim of this book is to provide the newcomer to these applications of NMR, whether a graduate student or a more senior biologist who wishes to apply NMR to his or her favourite protein, with practical guidance on how to choose the right experiment to obtain the desired information, how to carry out the experiment, and how to analyse the resulting spectra. Those who are familar with chemical applications of NMR but not those to biological macromolecules will also find this book
helpful in describing the special requirements of NMR studies of these large molecules. One of the consequences of the considerable proliferation of papers describing the application of NMR to biological macromolecules has been the increasing reluctance of journals to provide space for a detailed description of
the methods involved in, for example, resonance assignment or the analysis of NOEs for structure determination. This book will fill this gap, so as to allow the reader both to understand what is involved in the experiments described in the literature and to embark on the application of these powerful methods to his or her own system.
目次
- Introduction
- 1. Sample preparation
- 2. Instrumentation and pulse sequences
- 3. Resonance assignment strategies for small proteins
- 4. Stable isotope labelling and resonance assignments in larger proteins
- 5. Effects of chemical exchange on NMR spectra
- 6. NMR studies of protein-ligand interactions
- 7. NMR of nucleic acids from spectrum to structure
- 8. 1H NMR studies of oligosaccharides
- 9. Structure determination from NMR data I.
- 10. Analysis of NMR data
- 11. Structure determination from NMR data II.
- Computational approaches
- Appendix
- Index
- 巻冊次
-
: hbk ISBN 9780199632251
内容説明
Reflecting the increase in the use of nuclear magnetic resonance to study the conformations and interactions of biological macromolecules, this book provides guidance on how to choose the most appropriate protocol, how to carry out experiments and how to analyze the resulting spectra.
目次
- Sample preparation
- Instrumentation and pulse sequences
- Resonance assignment strategies for small proteins
- Stable isotope labelling and resonance assignments in larger proteins
- Effects of chemical exchange on NMR spectra
- NMR studies of protein-ligand interactions
- NMR of nucleic acids from spectrum to structure
- 1H NMR studies of oligosaccharides
- Structure determination from NMR data
- Analysis of NMR data
- Structure determination from NMR data
- Computational approaches.
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