Receptor autoradiography : principles and practice

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書誌事項

Receptor autoradiography : principles and practice

edited by John Wharton and Julia M. Polak

(Oxford science publications)

Oxford University Press, 1993

大学図書館所蔵 件 / 6

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注記

Includes bibliographical references

内容説明・目次

内容説明

Receptor autoradiography is a powerful aand robust technique that is widely used to localize and characterize peptide and neurotransmitter binding sites. Its extensive use reflects the utility of technique and the important advantages it offers over conventional biochemical procedures. This book aims to make receptor autoradiography more accesible by giving a detailed account of the methodology and its application in the analysis of receptors in the brain and peripheral organs. These examples are not exaustive but illustrate the versatility of the technique as well as its potential pitfalls. The book provides an introduction to the receptor concept and gives detailed informaitn on the theory and procedures involved in the auto radiographic localization and quantification of radiolabelled ligand binding sites. The book should be useful to neuroscientists, pharmacologists, histochemists, neuropathologists and scientists in the pharmaceuticals industry.

目次

  • An introduction to receptors, C.W. Taylor and S.C.R. Lummis
  • background to receptor mapping and future trends, M.J. Kuhar
  • analysis of receptors by radioligand binding, M. Keen and J. MacDermot
  • autoradiographic techniques, A.L. Hudson
  • in vitro autoradiographic localizaiton and characterization of binding sites, J. Wharton et al
  • quantitative analysis of radioligand binding autoradiographs, D.G. Baskin
  • autoradiographic localization of receptors at the electron microscopic level, A. Beaudet
  • analysis of receptor expression by in situ hybridization histochemistry, G. Mengod and and T. Vilaro
  • autoradiographic analysis of excitory amino-acid receptor, D.T. Monaghan
  • autoradiography of 5-HT receptors, C. Waeber and J.M. Palacios
  • autoradiographic visualization of dopaminergic receptors, M.S. Lidow
  • autoradiographic analysis of adenosine receptors, J.-L. Daval
  • neuropeptide and growth factor receptor autoradiography, R. Quiron et al
  • neuronal nicotinic acetycholine acetylcholine receptors, E.S. Deneris
  • localization and regulation of B-adrenoceptor subtypes.

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