In situ hybridization in neurobiology : advances in methodology
著者
書誌事項
In situ hybridization in neurobiology : advances in methodology
Oxford University Press, 1994
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
In situ hybridization has become an important and widely used research tool for neurobiologists. This new volume examines the methodological advances that have been made in in situ hybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs and both proteins and mRNAs within the same section, and methods for using in situ hybridization
technology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered. This volume will be essential to researchers in the
neurosciences as well as those in other disciplines.
目次
- 1. Methodological considerations in the utilization of In situ hybridization
- 2. Combining non-isotopic In situ hybridization histochemistry with steroid autoradiography
- 3. Development of techniques to combine isotopic and non-isotopic In situ hybridization and immunocytochemistry for phenotypic characterization of individual neurons
- 4. Enzyme histochemical detection of neuronal mRNA
- 5. Characterization of the receptor complement of individual neurons using dual-label In situ hybridization histochemistry
- 6. Assessment of peptide secretion and gene expression within individual cells
- 7. Quantitative micro-In situ hybridation
- 8. Quantification of In situ hybridization histochemistry
- 9. Analysis of transcription levels using intervening sequence In situ hybridization
- 10. Analysis of alternatively-transcribed messages in brain by In situ hybridization using biotinylated oligonucleotide probes
- 11. In situ polymerase chain reaction: A powerful new methodology
- 12. In situ transcription: Methodology and multi-faceted applications
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