Guide to techniques in glycobiology

Author(s)

• Lennarz, William J.
• Hart, Gerald W.

Bibliographic Information

Guide to techniques in glycobiology

edited by William J. Lennarz, Gerald W. Hart

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 230）

Academic Press, c1994

• : hard
• : pbk

Note

Includes bibliographical references and author and subject indexes

ISSN:0076-6879

Description and Table of Contents

Volume

: hard ISBN 9780121821319

Description

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Table of Contents

• Carbohydrate composition analysis of glycoconjugates by gas-liquid chromatography/mass spectrometry, R.K. Merkle and I. Poppe
• metabolic radiolabeling of glycoconjugates, A. Varki
• nonmetabolic radiolabeling and tagging of glyconconjugates, C.G. Gahmberg and M. Tolvanen
• enzymatic deglycosylation of asparagine-linked glycans - purification, properties, and specificity of oligosaccharide-cleaving enzymes from flavobacterium meningosepticum, A.L. Tarentino and T.H. Plummer
• release of oligosaccharides from glycoproteins by hydrazinolysis, T.P. Patel and R.B. Parekh
• use of lectins in analysis of glycoconjugates, R.C. Cummings
• saccharide linkage analysis using methylation and other techniques, R. Geyer and H. Geyer
• mass spectrometry of carbohydrate-containing bipolymers, A.Dell et al
• (1)H nuclear magnetic resonance spectroscopy of carbohydrate chains of glycoproteins, H. van Halbeek
• determination of stalic acids, G. Reuter and R. Schauer
• size fractionation of oligosaccharides, A. Kobata
• high-pH anion-exchange chromatography of glycoprotein-derived carbohydrates, M.R. Hardy and R.R. Townsend
• high-performance liquid chromatography of pyridylaminated saccharides, S. Hase
• high-performance liquid chromatography of oligosaccharides, J.E. Baenziger
• high-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides, P. Jackson
• capillary electrophoresis of oligosaccharides, R.J. Linhardt
• glycosidases in structural analysis, G.S. Jacob and P. Scudder
• glycosyltransferases in glycobiology, M.M. Palcic
• glycosidase inhibitors in study of glycoconjugates, G.P. Kaushal and A.D. Elbein
• synthesis and use of azido-substituted nucleoside diphosphate sugar photoaffinity analogs, A. Radominska and R.R. Drake
• glycoform analysis of glycoproteins, R.B. Parekh
• isolation of glycosphingolipids, R.L. Schnaar
• thin-layer chromatography of glycosphingolipids, R.L. Schnaar and L.K. Needham
• isolation and characterization of proteoglycans, V.C. Hascall et al
• structural analysis of glycosylphosphatidylinositol anchors, A.K. Menon
• detection of O-linked N-acetylglucosamine (O-GlcNAc) on cytoplasmic and nuclear proteins, E.P. Roquemore et al
• identification of polysialic acids in glycoconjugates, J. Ye et al
• neoglycolipds - probes of oligosaccharide structure, antigenicity, and function. T. Feizi et al.

Volume

: pbk ISBN 9780124436657

Description

Guide to Techniques in Glycobiology, designed to serve the needs of the student, experienced researcher, and newcomer to the field, presents in one source and format a wide variety of methods, each of wchich provides background material explaining the basis of the method followed by detailed procedures. Features: * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for carbohydrate chain analysis

Table of Contents

Carbohydrate Composition Analysis of Glycoconjugates By Gas-Liquid Chromatography. Mass Spectrometry. Metabolic Radiolabeling of Glycoconjugates. Nonmetabolic Radiolabeling and Tagging of Glyconconjugates. Enzymatic Deglycosylation of Asparagine-Linked Glycans: Purification, Properties and Specificity of Oligosaccharide-Cleaving Enzymes from Flavobacterium Meningosepticum. Release of Oligosaccharides from Glycoproteins By Hydrazinolysis. Use of Lectins in Analysis of Clycoconjugates. Saccharide Linkage Analysis Using Methylation and Other Techniques. Mass Spectrometry of Carbohydrates-Containing Bipolymers. 1H Nuclear Magnetic Resonance Spectroscopy of Carbohydrate Chains of Glycoproteins. Determination of Sialic Acids. Size Fractionation of Oligosaccharides. High-Ph Anion-Exchange Chromatography of Glycoprotein-Derived Carbohydrates. High-Performance Liquid Chromatography of Pyridylaminated Saccharides. High-Performance Liquid Chromatography of Oligosaccharides. High-Resolution Polycrylamide Gel Electro-Phoresis of Oligosaccharides. Glycosidases in Structural Analysis. Glycosyltransferases in Glycosidase Inhibitors in Study of Glycoconjugates. Synthesis and Use of Azido-Substituted Nucleoside Diphosphate Sugar Photoaffinity Analogs. Glycoform Analysis of Glycoproteins. Isolation of Glycosphingolipids. Thin-Layer Chromatography of Glycosphingolids. Isolation and Characterization of Proteoglycans. Structural Analysis of Glycosylphosphatidylinositol Anchors. Detection of O-Linked N-Acetylglucosamine (O-Glcnac) on Cytoplasmic and Nuclear Proteins. Identification of Polysialic Acids in Glycoconjugates. Neoglycolipds: Probes of Oligosaccharide Structure, Antigenicity, And Function. Author Index. Subject Index.

by "Nielsen BookData"

Details

• NCID: BA21717175
• ISBN:
  • 0121821315
  • 012443665X
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: San Diego ; Tokyo
• Pages/Volumes: xxvi, 567 p.
• Size: 24 cm
• Classification:
  • NLM : W1
• Subject Headings:
  • MESH : Glycoconjugates - analysis
  • MESH : Oligosaccharides - analysis
• Parent Bibliography ID:
  • BA00197543