Cellular interactions in development : a practical approach

著者

    • Hartley, David A.

書誌事項

Cellular interactions in development : a practical approach

edited by David A. Hartley

(The practical approach series, 131)

IRL Press at Oxford University Press, c1993

  • : hbk
  • : pbk

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注記

Includes bibliographical references and index

内容説明・目次

巻冊次

: pbk ISBN 9780199633906

内容説明

As progress in cell developmental biology carries on at a breakneck speed, new techniques constantly arise to plug the gaps left by traditional strategies. Cellular Interactions in Development provides detailed discussion and protocols of some of these new techniques, which allow the manipulation of developing organisms such as Drosophila or plants, when and where cells interact with each other to influence their development. The book looks at the really exciting innovations of the identification and functional test of molecules which control these cellular behaviours. The book also describes a number of new ways of hunting for these important proteins involved in cellular communication. A fully comprehensive manual which will prove indispensable to researchers in the fields of cell, developmental, and molecular biology.

目次

  • 1. Generating mosaics by site-specific recombinations
  • 2. Cell transplantation
  • 3. Clonal analysis in plants
  • 4. Toxin ablation in Drosophila
  • 5. Cloning cell surface molecules by transient expression
  • 6. Looking for protein-protein interactions
  • 7. Using the two-hybrid system to detect protein-protein interactions
  • 8. Purifying and assaying mesoderm-inducing factors from vertebrate embryos
  • Appendices
  • Index
巻冊次

: hbk ISBN 9780199633913

内容説明

As progress in cell developmental biology carries on at breakneck speed, new techniques constantly arise to plug the gaps left by traditional strategies. This work is aimed at an international audience of researchers, and contains a mixture of general discussion and experimental protocol. The techniques discussed cover a broad range of molecular and organismal biology, and allow the manipulation of developing organisms such as drosophila or plants when and where cells interact with each other to influence their development. The really exciting innovations are in the identification and functional test of the molecules which control these cellular behaviours. The book also describes new ways of hunting for these important proteins involved in cellular communication.

目次

  • Generating mosaics by site-specific recombination
  • cell transplantation
  • clonal analysis in plants
  • toxin ablation in drosophila
  • cloning cell surface molecules by transient expression
  • looking for protein-protein interactions
  • using the two-hybrid system to detect protein-protein interactions
  • purifying and assaying mesoderm-inducing factors from vertebrate embryos. Appendix: suppliers of specialist items.

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