Phenotyping and genotyping of intact cells
著者
書誌事項
Phenotyping and genotyping of intact cells
(The practical approach series, Diagnostic molecular pathology : a practical approach ; v. 1)
IRL Press, c1992
- : spiral hbk
- : pbk
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注記
Includes bibliographical references
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199632367
内容説明
Aimed at the pathologist specifically, this volume and its companion book bring together a broad spectrum of protocols relevant to the routine molecular analysis of pathological samples. It is written at a level which assumes little previous knowledge of the subject as many people working in clinical pathology laboratories have only limited experience of molecular techniques. Topics in Volume One include those methods which relate to morphological analysis, such as immunocytochemistry, "in situ" hybridization, chromosome analysis, and flow cytometry. Volume Two demonstrates additional applications of molecular techniques to clinical samples, including the use of PCR, DNA sequencing, and nucleic acid hybridization for such diverse purposes as pathogen identification and forensic studies. Expert guidance and detailed instruction on how to ensure accuracy and reliability for all the protocols is provided.
目次
- Molecular techniques in laboratory medicine, James O'D. McGee
- immunochemistry for light microscopy, Andrew R. Heryet and Kevin C. Gatter
- electron microscopical immunocytochemistry, Susan Van Noorden et al
- principles and basic methodology of DNA/RNA detection by "in situ" hybridization, C. Simon Herrington and James O'D. McGee
- viral gene detection by "in situ" hybridization, Stina M. Syrjanen
- interphase cytogenetics - an approach to the detection of genetic aberrations in tumours, Anton H.N. Hopman et al
- isotopic localization of cellular mRNA in clinical material using RNA probes, Q.A. Hamid
- mRNA detection in clinical samples by non-isotopic "in situ" hybridization, Eric P.-H. Yap et al
- "in situ" hybridization in diagnostic cytopathology, C.Simon Herrington and James O'D. McGee
- nucleolar organizer regions and fibrillar centres, John Crocker
- flow cytometry in clinical pathology,Richard S. Camplejohn
- list of contents of Volume II.
- 巻冊次
-
: spiral hbk ISBN 9780199632374
内容説明
Aimed at the pathologist specifically, this volume and its companion book bring together a broad spectrum of protocols relevant to the routine molecular analysis of pathological samples. It is written at a level which assumes little previous knowledge of the subject as many people working in clinical pathology laboratories have only limited experience of molecular techniques. Topics in Volume One include those methods which relate to morphological analysis, such as immunocytochemistry, "in situ" hybridization, chromosome analysis, and flow cytometry. Volume Two demonstrates additional applications of molecular techniques to clinical samples, including the use of PCR, DNA sequencing, and nucleic acid hybridization for such diverse purposes as pathogen identification and forensic studies. Expert guidance and detailed instruction on how to ensure accuracy and reliability for all the protocols is provided.
目次
- Molecular techniques in laboratory medicine, James O'D. McGee
- immunochemistry for light microscopy, Andrew R. Heryet and Kevin C. Gatter
- electron microscopical immunocytochemistry, Susan Van Noorden et al
- principles and basic methodology of DNA/RNA detection by "in situ" hybridization, C. Simon Herrington and James O'D. McGee
- viral gene detection by "in situ" hybridization, Stina M. Syrjanen
- interphase cytogenetics - an approach to the detection of genetic aberrations in tumours, Anton H.N. Hopman et al
- isotopic localization of cellular mRNA in clinical material using RNA probes, Q.A. Hamid
- mRNA detection in clinical samples by non-isotopic "in situ" hybridization, Eric P.-H. Yap et al
- "in situ" hybridization in diagnostic cytopathology, C.Simon Herrington and James O'D. McGee
- nucleolar organizer regions and fibrillar centres, John Crocker
- flow cytometry in clinical pathology, Richard S. Camplejohn
- list of contents of Volume II.
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