Cell and tissue genotyping

著者

書誌事項

Cell and tissue genotyping

Edited by C. S. Herrington andJ. O'D. McGee

（The practical approach series, Diagnostic molecular pathology : a practical approach ; v. 2）

IRL Press, c1992

  • : spiral hbk
  • : pbk

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注記

Includes bibliographical references

内容説明・目次

巻冊次

: pbk ISBN 9780199632381

内容説明

Molecular techniques are now being widely applied in clinical pathology laboratories, yet many people working in these facilities have only limited experience of such procedures. This work, together with Volume I, brings together a broad spectrum of protocols relevant to the routine molecular analysis of pathological samples. Volume I featured those methods which relate to morphological analysis, such as immunocytochemistry, in situ hybridization, chromosome analysis and flow cytometry. This second volume demonstrates additional applications of molecular techniques to clinical samples, including the use of PCR, DNA sequencing and nucleic acid hybridization for such diverse purposes as pathogen identification and forensic studies. For all the protocols, guidance and instructions are given on how to ensure accuracy and reliability, which should appeal to people working at all levels in apthology. The two volumes are also available as a set.

目次

  • V.T.-W. Chan: Extraction of nucleic acids from clinical samples and cultured cells
  • J.E. Stickland: Probe preparation and labelling
  • V. T.-W. Chan: Molecular hybridization of nucleic acids
  • D.K. Shibata: The polymerase chain reaction and the molecular genetic analysis of tissue biopsies
  • M.B. Llewelyn: DNA sequencing
  • H.M. Bauer & M.M. Manos: Determination of genital human papillomavirus infection of cells and tissues by generic polymerase chain reaction amplification
  • J.M.M. Walboomers, P.W.J. Melkert, A.J.C. van den Brule, P.J.F. Snijders & C.J.L.M. Meijer: The polymerase chain reaction for human papillomavirus screening in diagnostic cytopathology of the cervix
  • B.J. McCready & J.A. Chimera: Molecular detection and identification of pathogenic organisms
  • M. Eisenberg & J.A. Chimera: Human indentification by DNA analysis: the amplification fragment length polymorphism (AMPFLP) analysis technique
  • W. Yasui, H. Ito & E. Tahara: Analysis of archival material: application to tumour pathology
巻冊次

: spiral hbk ISBN 9780199632398

内容説明

Molecular techniques are now being widely applied in clinical pathology laboratories, yet many people working in these facilities have only limited experience of such procedures. This work, together with Volume I, brings together a broad spectrum of protocols relevant to the routine molecular analysis of pathological samples. Volume I featured those methods which relate to morphological analysis, such as immunocytochemistry, in situ hybridization, chromosome analysis and flow cytometry. This second volume demonstrates additional applications of molecular techniques to clinical samples, including the use of PCR, DNA sequencing and nucleic acid hybridization for such diverse purposes as pathogen identification and forensic studies. For all the protocols, guidance and instructions are given on how to ensure accuracy and reliability, which should appeal to people working at all levels in apthology. The two volumes are also available as a set.

目次

  • Extraction of nucleic acids from clinical samples and cultured cells, V.T-W. Chan
  • probe preparation and labelling, J.E. Stickland
  • molecular hybridization of nucleic acids, V.T-W. Chan
  • the polymerase chain reaction and the molecular genetic analysis of tissue biopsies, D.K. Shibata
  • DNA sequencing, M.B. Llewelyn
  • determination of genital human papillomavirus infection of cells and tissues by generic polymerase chain reaction amplification, H.M. Bauer and M.M. Manos
  • the polymerase chain reaction for human papillomavirus screening in diagnostic cytopathology of the cervix, J.M.M. Walboomers et al
  • molecular detection and identification of pathogenic organisms, B.J. McCready and J.A. Chimera
  • human identification by DNA analysis - the amplification fragment length polymorphism (AMPFLP) analysis technique, M. Eisenberg and J.A. Chimera
  • analysis of archival material - application to tumour pathology, W. Yasui et al.

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