Molecular imaging in neuroscience : a practical approach

書誌事項

Molecular imaging in neuroscience : a practical approach

edited by N.A. Sharif

(The practical approach series)

IRL Press at Oxford University Press, c1993

  • : hbk.
  • : pbk.

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注記

Includes bibliographical references and index

内容説明・目次

巻冊次

: pbk. ISBN 9780199633807

内容説明

This text provides protocols for the molecular imaging of genes, receptors, and receptor function in the nervous system. They represent the state of the art for studying brain function and dysfunction in the laboratory. It should be of interest to neuroscientists and pharmacologists, and is also suitable for advanced students and researchers in neuroscience, pharmacology, neuroanatomy, and neuropathology.

目次

  • 1. In situ hybridization histochemistry with radioactive and non-radioactive cRNA and DNA probes
  • 2. Comparison of the localization of protein kinase C subspecies by in situ hybridization and immunocytochemical methods
  • 3. Imaging gene expression in neural grafts
  • 4. Quantitative autoradiography: a tool to visualize and quantify receptors, enzymes, transporters, and second messenger systems
  • 5. Use of antibodies to visualize neurotransmitter receptor subtypes
  • 6. Immunofluorescent and immunogold methods to localize G proteins
  • 7. Technology for real time fluorescent ratio imaging in living cells using fluorescent probes for ions
  • 8. Flow cytometric selection of responsive subclones and fluorometric analysis of intracellular Ca - mobilization
  • Appendix
  • Index
巻冊次

: hbk. ISBN 9780199633814

内容説明

This text provides protocols for the molecular imaging of genes, receptors, and receptor function in the nervous system. They represent the state of the art for studying brain function and dysfunction in the laboratory. It should be of interest to neuroscientists and pharmacologists, and is also suitable for advanced students and researchers in neuroscience, pharmacology, neuroanatomy, and neuropathology.

目次

1: M.E. Lewis, E. Robbins and F. Baldino, Jr: In situ hybridization histochemistry with radioactive and non-radioactive cRNA and DNA probes. 2: J.F. McGinty, M.E. Couce, and D.K. Ways: Comparison of the localization of protein kinase C subspecies by in situ hybridization and immunocytochemical methods. 3: D.J.S. Sirinathsinghju and S.B. Dunnett: Imaging gene expression in neural grafts. 4: N.A. Sharif: Quantitative autoradiography: a tool to visualize and quantify receptors, enzymes, transporters, and second messenger systems. 5: A.I. Levey: Use of antibodies to visualize neurotransmitter receptor subtypes. 6: J.M. Lewis: Immunofluorescent and immunogold methods to localize G proteins. 7: W.T. Mason, J. Hoyland, I. Davison, M. Carew, J. Jonassen, R. Zorec, P.M. Lledo, G. Shankar, and M. Horton: Technology for real time fluorescent ratio imaging in living cells using fluorescent probes for ions. 8: J.T. Ranson, J.F. Dunne, and N.A. Sharif: Flow cytometric selection of responsive subclones and fluorometric analysis of intracellular Ca - mobilization. Appendix. Index

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