Methods for general and molecular bacteriology
著者
書誌事項
Methods for general and molecular bacteriology
American Society for Microbiology, c1994
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注記
Rev. ed. of: Manual of methods for general bacteriology
Includes bibliographical references and index
内容説明・目次
内容説明
A major revision of the classic manual from ASM. This is the long awaited revision of ASM's extremely popular title, Manual of Methods for General Bacteriology (1981). The goal of the book remains to provide a compact but thorough compendium of reliable methods of working with many different kinds of bacteria in laboratory settings. New to this edition is the recognition of the dramatic role of molecular biological techniques and their impact on bacteriology.
目次
- Methodology for General and Molecular Microbiology
- Morphology
- Light microscopy
- Determinative and cytological light microscopy
- Electron microscopy
- Cell fractionation
- Antigen-antibody reactions
- Growth: Physicochemical factors in growth
- Nutrition and media
- Enrichment and isolation
- Solid, liquid/solid and semisolid culture
- Liquid culture
- Growth measurement
- Culture preservation
- Molecular Genetics: Gene mutation
- Gene transfer in Gram-negative bacteria
- Gene transfer in Gram-positive bacteria
- Plasmids
- Transposon mutagenesis
- Gene cloning and expression
- Polymerase chain reaction
- Nucleic acid analysis
- Metabolism: Physical analysis
- Chemical analysis
- Enzymatic activity
- Permeability and transport
- Systematics: Phenotypic characterization
- DNA sequence similarities
- Ribosomal RNA hybridization and gene sequencing
- Nucleic acid probes
- General Methods: Laboratory safety
- Photography
- Records and reports
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