Male reproductive toxicology
著者
書誌事項
Male reproductive toxicology
（Methods in toxicology / series editors, Charles A. Tyson, Hanspeter Witschi, v. 3,
Academic Press, c1993
- : pbk
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内容説明・目次
- 巻冊次
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ISBN 9780124612075
内容説明
Methods in Toxicology, Volume 3: Male Reproductive Toxicology, Part A, deals with the male reproductive system and discusses methods that will help identify toxicant-induced changes at all levels in living organisms. It is important to realize that a toxic effect does not occur in a vacuum. All work in toxicology must be predicated on a demonstrated adverse effect in vivo. If good toxicology cannot exist in a vacuum, then there must be a structure. Thus, the book begins by presenting a few models as examples of the ways experiments could be grouped to define the toxicity of a chemical. This is followed by separate chapters on methods such as male mouse sexual behavior test; in vitro techniques for assessing pituitary secretory function; histological methods for preservation of the rat testis; procedures for assessing testicular sperm head counts in mice, rats, and dogs; and guidelines for conducting rodent dominant lethal tests. Subsequent chapters cover topics such as methods for the isolation and purification of Leydig cells from rat and mouse testes, and techniques used in semen analysis and fertility assessment in the rabbit.
目次
Contributors
1. Introduction
2. Male Mouse Sexual Behavior Test
3. In Vitro Techniques for Assessing Pituitary Secretory Function
4. Histological Methods for Evaluation of the Testis
5. Application of Testicular Sperm Head Counts in the Assessment of Male Reproductive Toxicity
6. Stage Synchronization in Rat Seminiferous Tubules Using Vitamin A Depletion and Repletion
7. Spermatogonial Stem Cells: Assessing Their Survival and Ability to Produce Differentiated Cells
8. Dominant Lethal Tests in Male and Female Mice
9. Transillumination-Phase-Contrast Microscopic Techniques for Evaluation of Male Germ Cell Toxicity and Mutagenicity
10. Isolation and Culture of Leydig Cells from Adult Rats
11. Isolation and Primary Culture of Leydig Cells
12. Purification and Primary Culture of Leydig Cells
13. Preparation and Use of Sertoli Cell-Enriched Cultures from 18-Day-Old Rats
14. Two-Compartment Cultures of Sertoli Cells-Applications in Testicular Toxicology
15. Isolation, Separation, and Short-Term Culture of Spermatogenic Cells
16. Collection of Interstitial Fluid and Seminiferous Tubule Fluid from the Rat Testis
17. Isolation and Culture of Epididymal Epithelial Cells from Adult Rats
18. Evaluation of Protein Synthesis by the Epididymis
19. Utilizing Cryo Resources CellSoft Computer-Assisted Sperm Analysis System for Rat Sperm Motility Studies
20. Computer-Assisted Sperm Analysis of Rodent Epididymal Sperm Motility Using the Hamilton-Thorn Motility Analyzer
21. Methods for Evaluation of Rat Epididymal Sperm Morphology
22. Semen Analysis and Fertility Assessment in the Rabbit
23* General Techniques for Assessing Male Reproductive Potential in Human Field Studies
Index
- 巻冊次
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: pbk ISBN 9780124612082
内容説明
In the tradition of "Methods in Enzymology" and "Methods in Neurosciences", Academic Press presents a new serial - "Methods in Toxicology". To address the needs of researchers in toxicology, toxicologic pathology, pharmacology, and clinical biochemistry, this new serial provides comprehensive descriptions of state-of-the-art methods for evaluating drug and chemical toxicity. Thematic volumes focus on mechanistic approaches to the study of toxicity both in vitro and in vivo, taking advantage of the recent advances in the biological and chemical sciences that allow closer scrutiny of the mechanisms by which chemical agents cause damage. Each volume begins with an introductory chapter that offers a broad guide to the application of methods addressed in that volume. Subsequent chapters contain detailed descriptions of research protocols, accessible both to experts and those new to toxicologic investigation. Included in each chapter are clearly defined procedures, discussions of limitations of the method, comparative considerations (species, sex, strain), interpretations of results, and explanations of how the methods may serve as alternatives to in vivo testing.
Each volume of "Methods in Toxicology" is available in case binding for the library and Wire-0 binding for the laboratory.
目次
R.E. Chapin and J.J. Heindel, Introduction. C. Chubb, Male Mouse Sexual Behaviour Test. M.D. Culler, In Vitro Techniques for Assessing Pituitary Secretory Function. R.A. Hess and B.J Moore, Histological Methods for Evaluation of the Testis. W.F. Blazak, KA. Treinen, and P.E. Juniewicz, The Application of Testicular Spermatid Head Counts in the Assessment of Male Reproductive Toxicity. C.C. Linder and M.D. Griswold, Stage Synchronization in Rat Seminiferous Tubules Using Vitamin A Depletion and Repletion. M.L. Meistrich and Maria E.A.B. van Beek, Spermatogonial Stem Cells: Assessing Their Survival and Ability to Produce Differentiated Cells. W.M. Generoso and W. W. Piegorsch, Dominant Lethal Tests in Male and Female Mice. M. Parvinen, J. Toppari, and J. Laehdetie, Transillumination-Phase Contrast Microscopic Techniques for Evaluation of Male Germ Cell Toxicity and Matagenicity. G.R. Klinefelter, W.R. Kelce, and M.P. Hardy, The Isolation and Culture of Leydig Cells from Adult Rats. L.B. Biegel J.C. Cook, and M.E. Hurtt, Isolation and Primary Culture of Leydig Cells. A.H. Payne and L. Sha, Purification and Primary Culture of Leydig Cells. W.W. Ku and R.E. Chapin, The Preparation and Use of Sertoli Cell-Enriched Cultures from 18-Day-Old Rats. A. Steinberger and J-P. Clinton, Two-Compartment Cultures of Sertoli Cells: Applications in Testicular Toxicology. D.A. O'Brien, Isolation, Separation, and Short-Term Culture of Spermatogenic Cells. G.L. Rehnberg, The Collection of Interstitial Fluid and Seminiferous Tubule Fluid from the Rat Testis. G.R. Klinefelter, The Isolation and Culture of Epididymal Epithelial Cells from Adult Rats. B.J Danzo, Evaluation of Protein Synthesis by the Epididymis. G.P. Toth, E.J. Read, and M.K. Smith, Utilizing Cryo Resources CellSoft Computer-Assisted Sperm Analysis System for Rat Sperm Motility Studies. V.L. Slott and S. D, Perreault, Computer-Assisted Sperm Analysis of Rodent Epididymal Sperm Motility Using the Hamilton-Thorn Motility Analyzer. R. Filler, Evaluation of Rat Epididymal Sperm Morphology. J. Williams, Semen Analysis and Fertility Assessment in the Rabbit. S.M. Schrader, General Techniques for Assessing Male Reproductive Potential in Human Field Studies.
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