RNA processing : a practical approach
著者
書誌事項
RNA processing : a practical approach
(The practical approach series, 135,
IRL Press at Oxford University Press, c1994
- v. 1 : pbk
- v. 1 : hbk
- v. 2 : pbk
- v. 2 : hbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
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v. 1 : pbk ISBN 9780199633432
内容説明
Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end-processing, capping, methylation, splicing, and editing
of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all the key steps required for a successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization
of specific RNAs, and the isolation and analysis of ribonucleoprotein complexes.
RNA processing - A Practical Approach, Volumes I and II, are invaluable laboratory companions for researchers working on mRNA, rRNA, or tRNA gene expression. Their lucid explanatory style and comprehensive coverage of all the key techniques for analysing RNA processing will be welcomed by both experienced researchers and
目次
- 1. Synthesis and purification of RNA substrates
- 2. Characterization of RNA
- 3. Splicing of mRNA precursors in mammalian cells
- 4. Isolation and characterization of ribonucleoprotein complexes
- 5. Analysis of ribonucleoprotein interactions
- 6. Analysis of pre-mRNA splicing in yeast
- Appendix
- 巻冊次
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v. 1 : hbk ISBN 9780199633449
内容説明
Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.
目次
- Synthesis and purification of RNA substrates
- Characterization of RNA
- Splicing of mRNA precursors in mammalian cells
- Isolation and characterization of ribonucleoprotein complexes
- Analysis of ribonucleoprotein interactions
- Analysis of pre-mRNA splicing in yeast
- Appendix
- 巻冊次
-
v. 2 : pbk ISBN 9780199634705
内容説明
Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full desciptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.
目次
- 1. 3'-end processing of mRNA
- 2. Capping and methylation of mRNA
- 3. RNA editing in mitochondria
- 4. Analysis of messenger RNA turnover in cell-free extracts from mammalian cells
- 5. Ribosomal RNA processing in vertebrates
- 6. Processing of transfer RNA precursors
- 7. Ribozymes
- Appendix
- 巻冊次
-
v. 2 : hbk ISBN 9780199634712
内容説明
Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full desciptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.
目次
- 3'-end processing of mRNA
- Capping and methylation of mRNA
- RNA editing in mitochondria
- Analysis of messenger RNA turnover in cell-free extracts from mammalian cells
- Ribosomal RNA processing in vertebrates
- Processing of transfer RNA precursors
- Ribozymes
- Appendix
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