Glycobiology : a practical approach
著者
書誌事項
Glycobiology : a practical approach
（The practical approach series）
IRL Press at Oxford University Press, c1993
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
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: pbk ISBN 9780199633715
内容説明
The interest in carbohydrates of glycoproteins has greatly intensified in the past few years. The recent development of recombinant glycoproteins for therapeutic purposes necessitates the understanding of the structure-function relationship in glycoproteins has significantly expanded. For instance, it is now known that carbohydrate-protein interaction is one of the most critical events in leukocytendothelial cell interaction. Such developments in biology and
medicine require an updated methodology that aims at the basic understanding of the structure and biosynthesis of glycoproteins. In this book, the chapters are assembled to provide basic background and methods in order to allow one to analyse the structures of glycoproteins. The subjects covered include
isolation, fractionation and structural characterization of typical glycoproteins, such as those containing N-glycans. The book also covers the phosphatidylinositol anchor, a new type of glycosylation to attach proteins to membranes.
目次
- 1. Isolation and characterization of glycoproteins
- 2. Compositional analysis of glycoproteins
- 3. Isolation and fractionation of N-linked oligosaccharides from glycoproteins
- 3a Release of N-linked oligosaccharides and fractionation by gel filtration
- 3b Fractionation of oligosaccharides by serial affinity chromatography with use of immobilized lectin columns
- 3c Fractionation of glycopeptides and oligosaccharides from glycoproteins by HPLC
- 4. Structural characterization of oligosaccharides from glycoproteins
- 4a Glycosidase treatment and other methods, including methylation analysis
- 4b FAM-MS and ES-MS of glycoproteins
- 4c Nuclear magnetic resonance spectroscopy of glycoprotein-derived carbohydrate chains
- 5. Structural characterization of N-glycans obtained from metabolically-radiolabelled glycoproteins
- 6. Structural characterization of mucin-type O-linked oligosaccharides
- 7. Characterization of nuclear and cytoplasmic glycoproteins
- 8. GPI membrane anchors
- Appendix
- Index
- 巻冊次
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ISBN 9780199633722
内容説明
An introduction to basic methods of analysing the structure of glycans attached to glycoproteins Comprehensive coverage - from simple methods to more sophisticated techniques The clear, step-by-step protocols are easy to follow and will enable the reader to determine the complete structure of glycans attached to proteins. With rapid development and heightened interest in carbohydrates of glycoproteins over recent years, this book provides an essential guide to this field for all levels of researchers. This book is intended for graduate students and post-doctoral researchers, in both an academic and industrial setting, who are interested in the analysis of carbohydrates and glycoproteins.
目次
- Isolation and characterization of glycoproteins
- Compositional analysis of glycoproteins
- Isolation and fractionation of N-linked oligosaccharides from glycoproteins
- Release of N-linked oligosaccharides and fractionation by gel filtration
- Fractionation of oligosaccharides by serial affinity chromatography with use of immobilized lectin columns
- Fractionation of glycopeptides and oligosaccharides from glycoproteins by HPLC
- Structural characterization of oligosaccharides from glycoproteins
- Glycosidase treatment and other methods, including methylation analysis
- FAM-MS and ES-MS of glycoproteins
- Nuclear magnetic resonance spectroscopy of glycoprotein-derived carbohydrate chains
- Structural characterization of N-glycans obtained from metabolically-radiolabelled glycoproteins
- Structural characterization of mucin-type O-linked oligosaccharides
- Characterization of nuclear and cytoplasmic glycoproteins
- GPI membrane anchors.
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