The Polymerase chain reaction
著者
書誌事項
The Polymerase chain reaction
Birkhäuser, c1994
- : us
- : us : pbk
- : gw
- : gw : pbk
- タイトル別名
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PCR
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: us ISBN 9780817636074
内容説明
Edited by the inventor of PCR and two prominent experts in PCR techniques, this first comprehensive handbook on the Polymerase Chain Reaction has the most up to date methodological protocols from the world's leading laboratories. Included are exciting new techniques and enhanced methods, previously unavailable in book form, that show the novice and experienced PCR user exactly how they can optimize their results. The applications chapters are quite unique, with the foremost researchers providing not only protocols, but explaining why PCR has revolutionized their particular field. Future enhancements of PCR as well as new potential uses are discussed. Readers will learn how PCR has changed the face of diagnostic testing, cancer research, genetics, forensics, plant biology, DNA sequencing, gene therapy, and much more! Nearly forty chapters have been extensively reviewed and checked for accuracy and breadth of subject matter.
目次
- Methodology
- manipulation of DNA, K. Hayashi
- cloning PCR products, M. Frohman
- multiplex PCRs, J.S. Chamberlain & J.R. Chamberlain
- preparation of nucleic acids, D. Shibata
- amplification of viral DNA and viral host cell mRNAs in situ, J. Embretson et al
- quantitation - an overview, F. Ferre et al
- quantification of DNAs by the polymerase chain using an internal control, D.M. Coen
- RT-PCR and mRNA quantitation, J. Chelly and A. Kahn
- analysis of T-cell repertoires by PCR, D. Spinella and J.M. Robertson
- ultra-sensitive non-radioactive detection of PCR products - an overview, R.H. Tullis
- fluorescent detection methods for PCR analysis, M.J. Heller
- enzyme-labeled oligonucleotides, E. Tu & E. Jablonski
- application of the hybridization protection assay to PCR, N.C. Nelson & S.H. McDonough
- instrumentation - an overview, C. Oste
- rapid cycle DNA amplification, C.T. Wittwer et al
- automating PCR, H.R. Gardner
- sequencing - an overview, R.A. Gibbs
- and applications chapters on PCR and cancer, diagnostics, gene therapy, plant biology, forensics, and much more.
- 巻冊次
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: us : pbk ISBN 9780817637507
内容説明
James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...
目次
One: Methodology.- I. Basic Methodology.- 1. Manipulation of DNA by PCR.- 2. Cloning PCR Products.- 3. Optimization of Multiplex PCRs.- 4. Preparation of Nucleic Acids for Archival Material.- 5. PCR Amplification of Viral DNA and Viral Host Cell mRNAs in Situ.- II. Quantitation.- 6. Quantitative PCR: An Overview.- 7. Quantification of DNAs by the Polymerase Chain Reaction Using an Internal Control.- 8. RT-PCR and mRNA Quantitation.- 9. Analysis of Human T-Cell Repertoires by PCR.- III. Nonisotopic Detection.- 10. Ultrasensitive Nonradioactive Detection of PCR Reactions: An Overview.- 11. Fluorescent Detection Methods for PCR Analysis.- 12. Enzyme-Labeled Oligonucleotides.- 13. Application of the Hybridization Protection Assay (HPA) to PCR.- IV. Instrumentation.- 14. PCR Instrumentation: Where Do We Stand?.- 15. Rapid Cycle DNA Amplification.- 16. Automating the PCR Process.- V. Sequencing.- 17. PCR and DNA Sequencing.- 18. Phage Promoter-Based Methods for Sequencing and Screening for Mutations.- 19. Capture PCR: An Efficient Method for Walking Along Chromosomal DNA and cDNA.- Two: Applications.- I. General Applications.- 20. In Vitro Evolution of Functional Nucleic Acids: High-Affinity RNA Ligands of the HIV-1 rev Protein.- 21. The Application of PCR to Forensic Science.- 22. Recreating the Past by PCR.- 23. Nonbiological Applications.- II. Genetic Analysis.- 24. RT-PCR and Gene Expression.- 25. Fingerprinting Using Arbitrarily Primed PCR: Application to Genetic Mapping, Population Biology, Epidemiology, and Detection of Differentially Expressed RNAs.- 26. Genetics, Plants, and the Polymerase Chain Reaction.- III. Assessment of Therapy Effectiveness.- 27. PCR Assessment of the Efficacy of Therapy in Philadelphia Chromosome-Positive Leukemias.- 28. The Detection of Minimal Residual Disease (MRD) in Acute Lymphoblastic Leukemia Using Clone-Specific Probes Directed against V(D)J Junctional Sequences.- 29. Assessment of Therapy Effectiveness: Infectious Disease.- 30. Gene Therapy.- IV. Diagnostics.- 31. PCR and Cancer Diagnostics: Detection and Characterization of Single Point Mutations in Oncogenes and Antioncogenes.- 32. Clinical Applications of the Polymerase Chain Reaction.- 33. Infectious Diseases.- Three: PCR and the World of Business.- 34. PCR in the Marketplace.- 35. PCR and Scientific Invention: The Trial of DuPont vs. Cetus.
- 巻冊次
-
: gw ISBN 9783764336073
内容説明
Edited by the inventor of PCR and two prominent experts in PCR techniques, this first comprehensive handbook on the Polymerase Chain Reaction has the most up to date methodological protocols from the world's leading laboratories. Included are exciting new techniques and enhanced methods, previously unavailable in book form, that show the novice and experienced PCR user exactly how they can optimize their results. The applications chapters are quite unique, with the foremost researchers providing not only protocols, but explaining why PCR has revolutionized their particular field. Future enhancements of PCR as well as new potential uses are discussed. Readers will learn how PCR has changed the face of diagnostic testing, cancer research, genetics, forensics, plant biology, DNA sequencing, gene therapy, and much more! Nearly forty chapters have been extensively reviewed and checked for accuracy and breadth of subject matter.
目次
- Methodology
- manipulation of DNA, K. Hayashi
- cloning PCR products, M. Frohman
- multiplex PCRs, J.S. Chamberlain & J.R. Chamberlain
- preparation of nucleic acids, D. Shibata
- amplification of viral DNA and viral host cell mRNAs in situ, J. Embretson et al
- quantitation - an overview, F. Ferre et al
- quantification of DNAs by the polymerase chain using an internal control, D.M. Coen
- RT-PCR and mRNA quantitation, J. Chelly and A. Kahn
- analysis of T-cell repertoires by PCR, D. Spinella and J.M. Robertson
- ultra-sensitive non-radioactive detection of PCR products - an overview, R.H. Tullis
- fluorescent detection methods for PCR analysis, M.J. Heller
- enzyme-labeled oligonucleotides, E. Tu & E. Jablonski
- application of the hybridization protection assay to PCR, N.C. Nelson & S.H. McDonough
- instrumentation - an overview, C. Oste
- rapid cycle DNA amplification, C.T. Wittwer et al
- automating PCR, H.R. Gardner
- sequencing - an overview, R.A. Gibbs
- and applications chapters on PCR and cancer, diagnostics, gene therapy, plant biology, forensics, and much more.
- 巻冊次
-
: gw : pbk ISBN 9783764337506
内容説明
Edited by the inventor of polymerase chain reaction (PCR) and the 1993 Nobel Prize winner in Chemistry, Kary Mullis, as well as two experts in the field, this handbook provides up-to-date methodological protocols from the world's leading laboratories, in addition to new techniques and enhanced applications not yet available in book form. Nearly 40 chapters inform the novice and experienced PCR user on how to optimize their results. In the chapters on applications, researchers provide not only protocols, but also descriptions of how PCR has revolutionized their particular field. Future enhancements of PCR, as well as new potential uses, are discussed. Readers can learn how PCR has changed the face of diagnostic testing, cancer research, genetics, forensics, plant biology, DNA sequencing and gene therapy. Special sections include the latest on QPCR, non-isotopic detection, genetic analysis, and PCR and the world of business, which includes a behind-the-scenes look at the legal battles between biotech giants Cetus and DuPont. It also provides insights into the origins of PCR and the history of nucleic acid research.
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