PCR technology : current innovations

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書誌事項

PCR technology : current innovations

edited by Hugh G. Griffin, Annette M. Griffin

CRC Press, c1994

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. A bestseller in its first edition, this second edition provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution.

目次

Building on the foundation of the bestselling first edition, this second edition supplies the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR, and explores preparative methods that address DNA generation for further analysis and in vitro evolution. It is a fundamental reference for those new to PCR and a source of up-to-date applications for those familiar with the method.

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