Thin-layer chromatography : techniques and applications

書誌事項

Thin-layer chromatography : techniques and applications

Bernard Fried, Joseph Sherma

(Chromatographic science series, v. 66)

Dekker, c1994

3rd ed., rev. and expanded

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内容説明・目次

内容説明

This title discusses all phases of thin-layer chromatography (TLC), including the preparation of biological samples prior to TLC, general practices of TLC, and applications based on compound types. Stresses practical rather than theoretical aspects of TLC, compares modern TLC with other separation methods, contains extensive coverage of sample preparation methods, surveys the latest instrumentation for high-performance and overpressured TLC, covers current principles and techniques, and more.

目次

  • Part 1 General practices of TLC: introduction and history
  • mechanism and theory
  • sorbents, layers, and precoated plates
  • obtaining material for TLC and sample preparation
  • application of samples
  • solvent systems
  • development techniques
  • detection and visualization
  • qualitative evaluation and documentation
  • quantification reproducibility of results
  • preparative TLC
  • radiochemical techniques. Part 2 Applications of TLC to different compound types. basic TLC design and TLC of organic dyes
  • lipids
  • amino acids
  • carbohydrates
  • natural pigments
  • vitamins
  • nucleic acid derivatives steroids and terpinoids
  • pharmaceuticals
  • miscellaneous
  • directory of manufacturers and suppliers of TLC instruments
  • plates and reagents.

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