Baculovirus expression vectors : a laboratory manual
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書誌事項
Baculovirus expression vectors : a laboratory manual
Oxford University Press, 1994
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注記
Originally published: W.H. Freeman, 1992
Includes bibliographical references and index
内容説明・目次
内容説明
This laboratory manual simplifies selection of the most appropriate baculovirus vector design for a given problem. It then describes each step of the implementation process - from vector construction to large-scale protein production. The book provides an understanding of how the vectors work, a biological overview of cells, viruses, plasmids, and promoters, guidelines for choosing optimum vectors, protocols for growing insect cells and recombinant viruses, methods
for analysing protein products and scaling up protein production, techniques for producing proteins in insect larvae, and easy to use maps charting available expression vectors.
目次
Acknowledgments
How To Use This Manual
PART I: AN OVERVIEW OF BACULOVIRUSES
1: Virus Structure and the Infection Process
2: Gene Organization, Regulation, and Function
3: Virus-Host Interactions
4: Summary of Baculovirus Features Relevant to Expression Factors
PART II: CHOOSING A TRANSFER PLASMID AND PARENT VIRUS
5: Choice of Virus and Host Species
6: Choice of Transfer Plasmid
7: Available Transfer Plasmids
8: Choosing a Parent Virus for Use in Vector Construction
9: Optimizing Expression: Tailoring the Heterologous Gene to the Transfer Plasmid and the Baculovirus Expression System
PART III: METHODS FOR VECTOR CONSTRUCTION AND GENE EXPRESSION
10: Safety Considerations
11: Insect Cell Culture
12: Virus Methods
13: Contransfection and Recombinant Virus Identification
14: Characterizing Recombinant Gene Expression
15: Post-Translational Modification
PART IV: METHODS FOR SCALE-UP OF PROTEIN PRODUCTION AND USE OF INSECT LARVAE
16: David L. Clemm: Scale-Up of Protein Production in a Stirred Bioreactor
17: Cheryl Isaac Murphy: Scale-Up of Protein Production in an Airlift Fermenter
18: Insect Rearing and Infection
Appendix 1: Nucleotide Sequence of AcMNPV Polyhedrin Region
Appendix 2: Nucleotide Sequence of AcMNPV p10 Region
Appendix 3: List of Genes Expressed in the Baculovirus Expression System
Appendix 4: Suppliers
Appendix 5: Stock Solutions
Appendix 6: Excel Spreadsheet for TCID[5[0 Calculation
References
Index
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