Flow cytometry : a practical approach
著者
書誌事項
Flow cytometry : a practical approach
（The practical approach series, 142）
IRL Press at Oxford University Press, c1994
2nd ed
- :hbk
- :pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
:pbk ISBN 9780199634613
内容説明
This is an updated edition of a laboratory manual which aims to provide biologists with an introduction to this relatively new technique. Leading experts offer valuable advice and guidance on this specialized form of microscopy, which is fast developing as an important application in modern hospital laboratories and biological and medical research laboratories. It should be beneficial to researchers in cell biology and biochemistry who already use flow cytometry, or who wish to use it for the first time.
目次
- Introduction to the principles of flow cytometry
- An introduction to fluorescence technology
- Preparing suspensions of single cells
- Flow sorting
- Immunofluorescence of surface markers
- Measurement of cytoplasmic and nuclear antigens
- Analysis of DNA - general methods
- Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method
- Analysis of cell proliferation using the bromodeoxyuridine-Hoechst ethidium bromide method
- Chromosome analysis and sorting
- Intracellular ionized calcium, magnesium, membrane potential and pH
- Flow cytometry of RNA content
- Measurement of dynamic cellular events
- Further applications to cell biology.
- 巻冊次
-
:hbk ISBN 9780199634620
内容説明
This is an updated edition of a laboratory manual which will assist biologists as an introduction to this relatively new technique. Leading experts offer valuable advice and guidance on this specialized form of microscopy, which is fast developing as an important application in modern hospital laboratories and biological and medical research laboratories. It should be beneficial to researchers in cell biology and biochemistry who already use flow cytometry, or who wish to use it for the first time.
目次
- Introduction to the principles of flow cytometry
- An introduction to fluorescence technology
- Preparing suspensions of single cells
- Flow sorting
- Immunofluorescence of surface markers
- Measurement of cytoplasmic and nuclear antigens
- Analysis of DNA - general methods
- Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method
- Analysis of cell proliferation using the bromodeoxyuridine-Hoechst ethidium bromide method
- Chromosome analysis and sorting
- Intracellular ionized calcium, magnesium, membrane potential and pH
- Flow cytometry of RNA content
- Measurement of dynamic cellular events
- Further applications to cell biology.
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