Molecular genetics of yeast : a practical approach
著者
書誌事項
Molecular genetics of yeast : a practical approach
（The practical approach series, 141）
IRL Press at Oxford University Press, c1994
- : hbk
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199634293
内容説明
This book describes and discusses experimental procedures used in a wide range of specialized topics within the genetics and molecular biology of the yeast Saccharomyces cerevisiae. Contributions are by internationally-recognized experts in particular areas and represent up-to-date methods currently in use. Topics covered include DNA isolation, cloning, and expression vectors, cosmid cloning, construction and use of cDNA libraries, plasmid shuffling and
mutant isolation, Ty insertional mutagenesis, high efficiency transformation, cell-free translation of mRNAs, Ty virus-like particles, and applications to industrial strains of yeast. Detailed protocols are clearly presented, with many additional practical tips, to assist both first-time and more experienced
experimentalists.
目次
- 1. Procedures for isolating yeast DNA for different purposes
- 2. Construction of cloning and expression vectors
- 3. Cosmid cloning of yeast DNA
- 4. The construction and use of cDNA libraries for genetic selections
- 5. Pulsed field gel electrophoresis
- 6. Plasmid shuffling and mutant isolation
- 7. Ty insertional mutagenesis
- 8. High efficiency transformation with lithium acetate
- 9. Measurement of transcription
- 10. Measurement of mRNA stability
- 11. Production of foreign proteins at high level
- 12. Cell-free translation of natural and synthetic mRNAs
- 13. Virus-like particles: Ty retrotransposons
- 14. The K1 killer toxin: molecular and genetic applications to secretion and cell surface assembly
- 15. Immuno-electron microscopy
- 16. Industrial Saccharomyces yeasts
- 巻冊次
-
: hbk ISBN 9780199634309
内容説明
The yeast Saccharomyces cerevisiae is used extensively in academic research and industry as a model eukaryote. It is easy to manipulate, is very flexible, and has a great many parallels with higher organisms. This makes it ideal for studies in molecular biology, genetics and biotechnology. This text describes current experimental procedures for working with the organism.
目次
1: Procedures for isolating yeast DNA for different purposes. 2: Construction of cloning and expression vectors. 3: Cosmid cloning of yeast DNA. 4: The construction and use of cDNA libraries for genetic selections. 5: Pulsed field gel electrophoresis. 6: Plasmid shuffling and mutant isolation. 7: Ty insertional mutagenesis. 8: High efficiency transformation with lithium acetate. 9: Measurement of transcription. 10: Measurement of mRNA stability. 11: Production of foreign proteins at high level. 12: Cell-free translation of natural and synthetic mRNAs. 13: Virus-like particles: Ty retrotransposons. 14: The K1 killer toxin: molecular and genetic applications to secretion and cell surface assembly. 15: Immuno-electron microscopy. 16: Industrial Saccharomyces yeasts
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