Principles of gene manipulation : an introduction to genetic engineering
Author(s)
Bibliographic Information
Principles of gene manipulation : an introduction to genetic engineering
（Studies in microbiology）
Blackwell Scientific, c1994
5th ed
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Note
Includes bibliographical references (p. 412-464) and index
Description and Table of Contents
Description
As in past editions, the basic philosophy of this extremely popular text remains to present the principles and techniques of gene manipulation in sufficient detail to enable the non-specialist reader to understand them. However, in the three years since the previous edition, molecular biology has seen a dramatic increase in available techniques and applications. Every chapter in this new edition has been extensively revised or rewritten to take these developments into account - including a vastly expanded section on the
Table of Contents
Introduction to Gene Manipulation Why do we clone genes? How do we clone genes?- the basic technique Cutting and joining DNA Cloning Genes in E. coli Plasmids as vectors Phage and cosmid vectors Cloning strategies, gene libraries and cDNA cloning Recombinant selection and screening Expression of cloned genes Analyzing DNA nucleotide sequences Changing genes - macro- and micro-modifications The polymerase chain reaction - an all embracing technique Impact of recombinant DNA on human genetics Cloning in Organisms other than E. coli Cloning in bacteria other than E. coli Cloning in yeast and other microbial eukaryotes Gene transfer to plants Introducing genes into animal cells Transferring genes into animal oocytes, eggs and embryos Biotechnology: The generation of novelty
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