DNA sequencing protocols
著者
書誌事項
DNA sequencing protocols
（Methods in molecular biology / John M. Walker, series editor, 23）
Humana Press, c1993
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting electrophoresis, sequencing of chemiluminescence, and automated sequencing.
目次
DNA Sequencing. M13 Cloning Vehicles: Their Contribution to DNA Sequencing. Cloning into M13. Transfection of E. coli with M13 DNA. M13 Phage Growth and Single Strand DNA Preparation. M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays. Generation of Random Fragments by Sonication. Generation of a Nested Set of Deletions Using Exonuclease III. Sequential Deletions of Single-Stranded DNA: Cyclone Sequencing. Subcloning for DNA Sequencing. Sequencing Using Custom Designed Oligonucleotides. Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1. Dideoxy Sequencing Reactions Using T7 Polymerase. Dideoxy Sequencing Reactions Using Sequenase Version 2.0. Dideoxy Sequencing Reactions Using Taq Polymerase. Pouring Linear and Buffer-Gradient Sequencing Gels. Electrophoresis of Sequence Reaction Samples. Plasmid Sequencing. Direct Sequencing of Inserts Cloned into Lambda Vectors. Cosmid Sequencing. Genomic Sequencing. Sequencing of Double-Stranded PCR Products. Sequencing Double-Stranded Linear DNA with Sequenase and [a-35S]-dATP. Solid Phase PCR Sequencing of Biotinylated Products. Cycle Sequencing. Direct Blotting Electrophoresis. Multiplex DNA Sequencing. DNA Sequencing by Chemiluminescent Detection. Reverse Sequencing of M13 Cloned DNA. Terminal Labeling of DNA for Maxam and Gilbert Sequencing. DNA Sequencing by the Chemical Method. DNA Sequencing by Chemical Degradation Using One Two and Four Different Fluorophores. Linear Amplification Sequencing with Dye Terminators. Sequencing Reactions for the Applied Biosystems 373A Automated Sequencer. Sequencing Reactions for the ALF (EMBL) Automated Sequencer. Sequencing Using the DuPont Genesis% 2000 DNA Analysis System. The Use of Robotic Workstations in DNA Sequencing. Index.
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