書誌事項

Flow cytometry

edited by Zbigniew Darzynkiewicz, J. Paul Robinson, Harry A. Crissman

(Methods in cell biology / edited by David M. Prescott, v. 41, 42)

Academic Press, c1994

2nd ed

  • pt. A : hbk
  • pt. A : pbk
  • pt. B : hbk
  • pt. B : pbk

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注記

Includes bibliographical references and index

内容説明・目次

巻冊次

pt. A : pbk ISBN 9780122030512

内容説明

Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.

目次

  • Dissociation of intact cells from tumours and normal tissues, D.W. Visscher and J.D. Crissman
  • assays of cell viability - discrimination of cells dying by apoptosis, Z. Darzynkiewicz et al
  • cell preparation for the identification of leukocytes, C.C. Stewart
  • multiparameter analysis of leukocytes by flow cytometry, C.C. Stewart
  • immunophenotyping using fixed cells, G.F. Babcock and S.M. Dawes
  • simultaneous DNA content and cell surface immunofluorescence analysis, P.P.T. Brons et al
  • flow cytometry crossmatching for solid organ transplantation, R.A. Bray
  • cell membrane potential analysis, H.M. Shapiro
  • measurement of intracellular pH, M.J. Boyer and D.W. Hedley
  • intracellular ionized calcium, C.H. June and P.S. Rabinovitch
  • cellular protein content measurements, H.A. Crissman and J.A. Steinkamp
  • lysosomal proton pump activity - supravital cell staining with acridine orange differentiates leukocyte subpopulations, F. Traganos and Z. Darzynkiewicz
  • staining of DNA in live and fixed cells, H.A. Crissman and G.T. Hirons
  • high-resolution analysis of nuclear DNA employing the fluorochrome DAPI, F.J. Otto
  • detergent and proteolytic enzyme based techniques for nuclear isolation and DNA content analysis, L.L. Vindelov and I.J. Christensen
  • DNA analysis from paraffin-embedded blocks, D.W. Hedley
  • controls, standards and histogram interpretation in DNA flow cytometry, L.G. Dressler and L.C. Seamer
  • DNA content histogram and cell cycle analysis, P.S. Rabinovitch
  • immunochemical quantitation of bromodeoxyuridine - application to cell cycle kinetics, F. Dolbeare and J.R. Selden
  • application and detection of IdUrd and CldUrd as two independent cell cycle markers, J.A. Aten et al
  • cell cycle analysis using continuous bromodeoxyuridine labelling and Hoechst 33358-ethidium bromide bivariate flow cytometry, M. Poot et al
  • detection of BrdUrd-labelled cells by differential analysis of DNA fluorochromes - pulse-chase and continuous labelling methods, H.A. Crissman et al
  • analysis of intracellular proteins, K.D. Bauer and J.W. Jacobberger
  • "washless" procedures for nuclear antigen detection, J.K. Larsen
  • light scatter of isolated cell nuclei as a parameter discriminating the cell cycle subcompartments, W. Giaretti and M. Nusse
  • simultaneous analysis of cellular RNA and DNA content, Z. Darzynkiewicz
  • analysis of DNA content and cyclin proteins expression in studies of DNA ploidy, growth fraction, lymphocyte stimulation and cell cycle, Z. Darzynkiewicz et al
  • oxidative product formation analysis by flow cytometry, J.P. Robinson et al
  • flow cytometric determination of cysteine and serine proteinase activities in living cells with rhodamine 110 substrates, S. Klingel et al
  • leucine aminopeptidase activity by flow cytometry, J.J. Turek and J.P. Robinson
  • enzyme kinetics, J.V. Watson and C. Dive
  • on-line flow cytometry - a versatile method for kinetic measurements, K. Nooter et al. (Part contents).
巻冊次

pt. B : pbk ISBN 9780122030529

内容説明

Flow Cytometry, Second Edition is a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow cytometry to separate and analyze cells.

目次

  • A flow cytometric technique for detection of DNA repair in mammalian cells, J.R. Selden and F. Dolbeare
  • rapid determination of cellular resistance related drug efflux in tumour cells, A. Krishan
  • glutathione and cellular resistance to anti-cancer drugs, D. Hedley and S. Chow
  • assay of cell resistance to Ara-C, F. Lacombe
  • detection of mRNA species by flow cytometry, F. Belloc
  • PRimed IN Situ labelling (PRINS) and Fluorescent In Situ Hybridization (FISH), H. Fischer et al
  • molecular phenotyping by flow cytometry, B.D. Li et al
  • isolation and analysis of somatic cell mutants with defects in endocytic traffic, S.A. Brockman and R.F. Murphy
  • measurement of micronuclei by flow cytometry, M. Nusse et al
  • sperm chromatin structure assay (DNA denaturability), D. Evenson and L. Jost
  • fine-needle cytopuncture and flow cytometry in the characterization of human tumours, F. Spyratos and M. Briffod
  • functional NK assay using flow cytometry, S. Papa and M. Valentini
  • antibodies to intermediate filament proteins as probes for multi-parameter flow cytometry of human cancers, T.V. Shankey
  • interactive data analysis for evaluation of B-cell neoplasia by flow cytometry, R.E. Duque
  • analysis and sorting of hematopoietic stem cells from mouse bone marrow, J.M.W. Visser and P. de Vries
  • reticulocyte analysis and reticulocyte maturity index, B.H. Davis and N.C. Bigelow
  • analysis of platelets by flow cytometry, K.A. Ault and J. Mitchell
  • flow cytometry in malaria detection, C.J. Janse and P.H. Van Vianen
  • large-scale chromosomes sorting, J.J. Fawcett et al
  • strategies for rare cell detection and isolation, J.F Leary
  • cell sorting of biohazardous specimens for assay of immune function, J.V. Giorgi
  • high-speed photodamage cell sorting - an evaluation of the ZAPPER prototype, J.F. Keij et al
  • high-gradient magnetic cell sorting, A. Radbruch et al
  • contributions of flow cytometry to studies with multicell spheroids, R.E. Durand
  • functional measurements using HL-60 cells, J.P. Robinson et al
  • HIV infection - diagnosis and disease progression evaluation, J.V. Giorgi and A. Landay
  • cell cycle analysis of "saccharomyces cerevisiae", B.S. Dien et al
  • staining and measurement of DNA in bacteria, H.B. Steen et al
  • detection of specific microorganisms, G. Vesey et al
  • strategies for flow cytometric analysis of marine microalgae and sponge cells, C.M. Yentsch and S.A. Pomponi
  • flow cytometry and sorting of plant protoplasts and cells, D.W. Galbraith
  • use of fluorescence-activated cell sorting for rapid isolation of insect cells harbouring recombinant baculovirus, M.A. Sommerfelt and E.J. Sorscher
  • flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes, T.M. McHugh
  • calibration of flow cytometer detector systems, R.E. Durand. (Part contents).
巻冊次

pt. A : hbk ISBN 9780125641425

内容説明

Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.

目次

D.W. Visscher and J.D. Crissman, Dissociation of Intact Cells from Tumors and Normal Tissues. Z. Darzynkiewicz, X. Li, and J. Gong, Assays of Cell Viability: Discrimination of Cells Dying by Apoptosis. C.C. Stewart and S.J Stewart, Cell Preparation for the Identification of Leukocytes. C.C. Stewart and S.J. Stewart, Multiparameter Analysis of Leukocytes by Flow Cytometry. G.F. Babcock and S.M. Dawes, Immunophenotyping Using Fixed Cells. P.P.T.Brons, P.E.J. Van Erp, and A.H.M. Pennings, Simultaneous DNA Content and Cell-Surface Immunofluorescence Analysis. H.M. Shapiro, Cell Membrane Potential Analysis. M.J. Boyer and D.W. Hedley, Measurement of Intracellular pH. C.H. June and P.S. Rabinovitch, Intracellular Ionized Calcium. H.A. Crissman and J.A. Steinkamp, Cellular Protein Content Measurements. F. Traganos and Z. Darzynkiewicz, Lysosomal Proton Pump Activity: Supravital Cell Staining with Acridine Orange Differentiates Leukocyte Subpopulations. H.A. Crissman and G.T. Hirons, Staining of DNA in Live and Fixed Cells. F.J. Otto, High-Resolution Analysis of Nuclear DNA Employing the Fluorochrome DAPI. L.L. Vindelov and I.J. Christensen, Detergent and Proteolytic Enzyme-Based Techniques for Nuclear Isolation and DNA Content Analysis. D.W. Hedley, DNA Analysis from Paraffin-Embedded Blocks. L.G. Dressler and L.C. Seamer, Controls, Standards, and Histogram Interpretation in DNA Flow Cytometry. P.S. Rabinovitch, DNA Content Histogram and Cell-Cycle Analysis. F. Dolbeare and J.R. Selden, Immunochemical Quantitation of Bromodeoxyuridine: Application to Cell-Cycle Kinetics. J.A. Aten, J. Stap, R. Hoebe, and P.J.M. Bakker, Application and Detection of IdUrd and CldUrd as Two Independent Cell-Cycle Markers. M. Poot, H. Hoehn, M. Kubbies, A. Grossmann, Y. Chen, and P.S. Rabinovitch, Cell-Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33358--Ethidium Bromide Bivariate Flow Cytometry. H.A. Crissman, N. Oishi, and R. Habbersett, Detection of BrdUrd-Labeled Cells by Differential Fluorescence Analysis of DNA Fluorochromes: Pulse-Chase and Continuous Labeling Methods. K.D. Bauer and J.W. Jacobberger, Analysis of Intracellular Proteins. J.K. Larsen, "Washless" Procedures for Nuclear Antigen Detection. W. Giaretti and M. Nusse, Light Scatter of Isolated Cell Nuclei as a Parameter Discriminating the Cell-Cycle Subcompartments. Z. Darzynkiewicz, Simultaneous Analysis of Cellular RNA and DNA Content. Z. Darzynkiewicz, J. Gong, and F. Traganos, Analysis of DNA Content and Cyclin Protein Expression in Studies of DNA Ploidy, Growth Fraction, Lymphocyte Stimulation, and the Cell-Cycle. J.P. Robinson, W.O. Carter, and P.K. Narayanan, Oxidative Product Formation Analysis by Flow Cytometry. S. Klingel, G. Rothe, W. Kellermann, and G. Valet, Flow Cytometric Determination of Cysteine and Serine Proteinase Activities in Living Cells with Rhodamine 110 Substrates. J.J. Turek and J.P. Robinson, Leucine Aminopeptidase Activity by Flow Cytometry. J.V. Watson and C. Dive, Enzyme Kinetics. K. Nooter, H. Herweijer, R.R. Jonker, and G.J. van den Engh, On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements. Z. Darzynkiewicz, Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin Structure. J. Laffin and J.M. Lehman, Detection of Intracellular Virus and Viral Products. Index. Volumes in Series.
巻冊次

pt. B : hbk ISBN 9780125641432

内容説明

This is the second of a two volume set that provides a laboratory guide and reference on methods in flow cytometry sample preparation and analysis. The theoretical foundations, critical aspects and possible pitfalls of each procedure is outlined, and protocols are supplemented with explanatory sections and trouble-shooting tips. Various cell systems are described and ten methods cover assessment of apoptosis and other modes of cell death. The methods detailed should be accessible to researchers and students in biomedical science and biology who use flow cytometry to separate and analyze cells.

目次

  • A flow cytometric technique for detection of DNA repair in mammalian cells, J.R. Selden and F. Dolbeare
  • rapid determination of cellular resistance related drug efflux in tumour cells, A. Krishan
  • glutathione and cellular resistance to anti-cancer drugs, D. Hedley and S. Chow
  • assay of cell resistance to Ara-C, F. Lacombe
  • detection of mRNA species by flow cytometry, F. Belloc
  • PRimed IN Situ labelling (PRINS) and Fluorescent In Situ Hybridization (FISH), H. Fischer et al
  • molecular phenotyping by flow cytometry, B.D. Li et al
  • isolation and analysis of somatic cell mutants with defects in endocytic traffic, S.A. Brockman and R.F. Murphy
  • measurement of micronuclei by flow cytometry, M. Nusse et al
  • sperm chromatin structure assay (DNA denaturability), D. Evenson and L. Jost
  • fine-needle cytopuncture and flow cytometry in the characterization of human tumours, F. Spyratos and M. Briffod
  • functional NK assay using flow cytometry, S. Papa and M. Valentini
  • antibodies to intermediate filament proteins as probes for multi-parameter flow cytometry of human cancers, T.V. Shankey
  • interactive data analysis for evaluation of B-cell neoplasia by flow cytometry, R.E. Duque
  • analysis and sorting of hematopoietic stem cells from mouse bone marrow, J.M.W. Visser and P. de Vries
  • reticulocyte analysis and reticulocyte maturity index, B.H. Davis and N.C. Bigelow
  • analysis of platelets by flow cytometry, K.A. Ault and J. Mitchell
  • flow cytometry in malaria detection, C.J. Janse and P.H. Van Vianen
  • large-scale chromosomes sorting, J.J. Fawcett et al
  • strategies for rare cell detection and isolation, J.F Leary
  • cell sorting of biohazardous specimens for assay of immune function, J.V. Giorgi
  • high-speed photodamage cell sorting - an evaluation of the ZAPPER prototype, J.F. Keij et al
  • high-gradient magnetic cell sorting, A. Radbruch et al
  • contributions of flow cytometry to studies with multicell spheroids, R.E. Durand
  • functional measurements using HL-60 cells, J.P. Robinson et al
  • HIV infection - diagnosis and disease progression evaluation, J.V. Giorgi and A. Landay
  • cell cycle analysis of "saccharomyces cerevisiae", B.S. Dien et al
  • staining and measurement of DNA in bacteria, H.B. Steen et al
  • detection of specific microorganisms, G. Vesey et al
  • strategies for flow cytometric analysis of marine microalgae and sponge cells, C.M. Yentsch and S.A. Pomponi
  • flow cytometry and sorting of plant protoplasts and cells, D.W. Galbraith
  • use of fluorescence-activated cell sorting for rapid isolation of insect cells harbouring recombinant baculovirus, M.A. Sommerfelt and E.J. Sorscher
  • flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes, T.M. McHugh
  • calibration of flow cytometer detector systems, R.E. Durand. (Part contents).

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  • Methods in cell biology

    edited by David M. Prescott

    Academic Press 1964-

    v. 1 , v. 2 , v. 3 , v. 4 , v. 5 , v. 6 , v. 7 , v. 8 , v. 9 , v. 10 , v. 11 , v. 12 , v. 13 , v. 14 , v. 15 , v. 20 , v. 21A , v. 21B , v. 24 , v. 25 , v. 26 , v. 27 , v. 28 , v. 29 , v. 30 , v. 31 , v. 32 , v. 33 , v. 34 , v. 35 , v. 36 , v. 40 , v. 44 , v. 54 , v. 72 , v. 73

    所蔵館65館

詳細情報

  • NII書誌ID(NCID)
    BA23846113
  • ISBN
    • 0125641427
    • 0122030516
    • 0125641435
    • 0122030524
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    San Diego ; New York
  • ページ数/冊数
    2 v.
  • 大きさ
    24 cm
  • 件名
  • 親書誌ID
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