Keratinocyte methods

著者

書誌事項

Keratinocyte methods

edited by Irene M. Leigh and Fiona M. Watt

Cambridge University Press, 1994

大学図書館所蔵 件 / 4

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Keratinocytes, as the main cellular component of the organism/environment interface, perform a vast range of functions in protection, secretion, sensation and self-repair by virtue of their great plasticity of form and development. Indeed recent medical advances in laboratory culture of these cells for use as skin grafts in cases of severe burns or ulceration owe much of their success to this very plasticity. This very practical handbook draws upon a wide range of international expertise making a collection of methods suitable for keratinocyte manipulation for use by new and old hands alike. Together with its companion volume The Keratinocyte Handbook it provides a comprehensive dossier of the keratinocyte which will prove an invaluable reference resource for all encountering keratinocytes in both laboratory and clinic, including dermatologists, molecular and cell biologists and biochemists.

目次

  • 1. CULTURE TECHNIQUES AND GRAFTING METHODS: introduction
  • 2. Human epidermal keratinocytes
  • 3. Mouse epidermal keratinocytes
  • 4. Specialized organ cultures
  • 5. Skin equivalents
  • 6. Keratinocytes for grafting
  • 7. ASSAYS OF KERATINOCYTE ADHESION, PROLIFERATION AND GROWTH FACTOR PRODUCTION
  • 8. METHODS FOR STUDYING DIFFERENT SUBPOPULATIONS OF KERATINOCYTES
  • 9. Markers for terminal differentiation
  • 10. Introduction of foreign genes into keratinocytes
  • References.

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