Plant cell culture : a practical approach

書誌事項

Plant cell culture : a practical approach

edited by Richard A. Dixon and Robert A. Gonzales

(The practical approach series, 145)

IRL Press at Oxford University Press, c1994

2nd ed

  • : pbk

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注記

Includes bibliographical references and index

内容説明・目次

巻冊次

: pbk ISBN 9780199634026

内容説明

Plant cell culture techniques are being increasingly used in basic research and for plant exploitation in industry (genetic engineering, micropropagation). In this book, internationally recognized experts describe the techniques in use in their laboratories for isolating and manipulating cell cultures, and their central importance in plant biotechnology. This book is intended for postgraduate researchers, college lecturers, industrial/biotechnology researchers in plant sciences.

目次

  • 1. Isolation and maintenance of callus and cell suspension cultures
  • 2. Isolation, culture, and regeneration of protoplasts
  • 3. Applications of protoplast technology
  • 3.A Fusion and selection of somatic hybrids
  • 3.B Transient gene expression and stable transformation
  • 3.C Studies with viruses
  • 4. Selection of plant cells for desirable characteristics
  • 4.A Inhibitor resistance
  • 4.B Cold tolerance
  • 4.C In vitro selection for salt tolerance
  • 4.D In vitro selection for disease/toxin resistance
  • 5. Plant regeneration via embryonic suspension cultures
  • 6. Applied aspects of plant regeneration
  • 6.A Micropropagation
  • 6.B Virus-free plants
  • 6.C Artificial seeds
  • 7. Cryopreservation
  • 8. Secondary products from cultured cells and organs I: molecular and cellular approaches
  • 9. Secondary products from cultured cells and organs II: large scale culture
巻冊次

ISBN 9780199634033

内容説明

Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including genetic engineering and micropropagation, and also from a crucial element in the new transgenic technology. This new edition covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. Thoroughly revised, it retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, plus an appeal to a broad readership, that made the first edition so successful. This book is intended for postgraduate researchers, lecturers and industrial and biotechnology researchers in plant sciences.

目次

1: Isolation and maintenance of callus and cell suspension cultures. 2: Isolation, culture, and regeneration of protoplasts. 3: Applications of protoplast technology. 3.A: Fusion and selection of somatic hybrids. 3.B: Transient gene expression and stable transformation. 3.C: Studies with viruses. 4: Selection of plant cells for desirable characteristics. 4.A: Inhibitor resistance. 4.B: Cold tolerance. 4.C: In vitro selection for salt tolerance. 4.D: In vitro selection for disease/toxin resistance. 5: Plant regeneration via embryonic suspension cultures. 6: Applied aspects of plant regeneration. 6.A: Micropropagation. 6.B: Virus-free plants. 6.C: Artificial seeds. 7: Cryopreservation. 8: Secondary products from cultured cells and organs I: molecular and cellular approaches. 9: Secondary products from cultured cells and organs II: large scale culture

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