Analytical biochemistry
著者
書誌事項
Analytical biochemistry
Longman Scientific & Technical, 1993 , Copublished in the U.S. with Wiley, 1993
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
The objective was to bring together in one book those topics which the authors consider to be essential to the subject of analytical biochemistry. In the introductory section to each chapter, there is a brief explanation of the scientific basis of the topic and this is followed by a discussion of the analytical methods which are relevant. While it is not intended that it should be a book of "recipes", technical details for many of the methods described are given. It is intended that the book should provide enough information to enable a student to select a technique or series of techniques which would be appropriate for a particular analytical problem and to be able to develop a valid and reliable analytical method. The topics covered in the book fall into three main groups. Analytical techniques such as spectroscopy, chromatography, etc. are particularly important in analytical biochemistry as well as in analytical chemistry generally. The principles of each technique are explained and the scope and applications are discussed.
There are chapters on enzymes, antibodies and radio-isotopes, substances which it may be necessary to detect and measure but which also can be very useful in a variety of analytical methods. Here again, the basic theory is explained before discussing their applications in analytical biochemistry. Finally, there are four chapters which explain the chemical nature and methods of analysis of the major groups of biologically important compounds, namely, carbohydrates, amino acids, proteins and lipids. At the end of each chapter, several books are listed for further reading.
目次
- Part 1 General principles of analytical biochemistry: the selection of a valid method of analysis
- the quality of data
- the presentation of data. Part 2 Spectroscopy: interaction of radiation with matter
- molecular absorptiometry
- absorptionmeter design
- molecular fluorescence techniques
- atomic spectroscopy techniques
- magnetic resonance spectroscopy. Part 3 Separation techniques: principles of separation techniques
- methods based on polarity
- methods based on ionic nature
- methods based on size
- methods based on shape. Part 4 Electroanalytical methods: poteniometry
- conductimetry
- coulometry
- voltammetry
- biosensors. Part 5 Radio-isotopes: nature of radioactivity
- detection and measurement of radioactivity
- biochemical uses of isotopes. Part 6 Automated methods of analysis: discrete analyzers
- flow analysis
- robotics. Part 7 Immunological methods: general processes of the immune response
- antigen - antibody reactions
- analytical techniques - precipitation reactions
- analytical techniques - immunoassay. Part 8 Enzymes: the nature of enzymes
- monitoring techniques
- enzyme assay methods
- substrate assay methods
- immobilized enzymes
- treatment of cells and tissues for enzyme assay. Part 9 Carbohydrates: general structure and function
- chemical methods of carbohydrate analysis
- enzymic methods of carbohydrate analysis
- separation and identification of carbohydrate mixtures. Part 10 Aminno acids: general structure and properties
- general reactions
- N-terminal analysis
- reactions of specific amino acids
- separation of amino acid mixtures
- amino acid analyzer. Part 11 Proteins: protein structure
- general methods of quantitation
- separation of proteins. Part 12 Lipids: fatty acids
- simple lipids
- complex lipids
- lipid-protein structures
- sample preparation and handling
- quantitative methods
- separation of lipid mixtures. Part 13 Nucleic acids: nucleic acid composition and structure
- isolation and purification of nucleic acids
- methods of nucleic acid analysis
- vectors
- DNA sequencing. Part 14 Self-test questions and problems.
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