Haematological cytochemistry
著者
書誌事項
Haematological cytochemistry
Churchill Livingstone, 1994
3rd ed
大学図書館所蔵 全9件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
注記
Includes bibliographical references and index
内容説明・目次
内容説明
The second edition of this book, published in 1988, incorporated over four thousand new references to advances in cytochemical practice covering predominantly orthodox applications of chemical colour reactions in the direct demonstration of intracellular constituents, including a wide range of enzymes, but also dealing with the growing science of immunocytochemistry. During the years preceding the third edition certain refinements of standard cytochemical procedures, including improvements in quantitative methods and approaches, and some additional techniques of value, such as the important silver staining methods for nucleolar organizer regions, have been introduced and are described and discussed appropriately, but the main bulk of the 600 further new references now incorporated in this edition are concerned with the fields of immunocytochemistry and the still more recent but rapidly expanding science of hybridocytochemistry.
The impact of immnocytochemistry in the study of haemic cells and especially in the differential diagnosis of subvariants of leukaemias, lymphomas and allied malignant states has warranted further considerable expansion of Chapter 3, where the general principles of immunocytochemistry are discussed, and Chapters 14 and 15, where the overall cytochemical findings in these neoplastic diseases are brought together. The increased use of flow cytofluorometric techniques to identify and separate components of mixed cell populations reacting with different surface antigen reactions, and the potential combination of DNA quantitation with such marker reactions, are discussed and illustrated. The development of antibodies, either polyclonal or monoclonal, against antigen epitopes resistant to standard fixation and embedding procedures used in histology has allowed the extension of surface marker discrimination from cell suspensions and smear preparations to formalin-fixed and paraffin wax-embedded sections of bone marrow or lymph node biopsies, and the nature and result of such advances are discussed.
The techniques of in situ hybridization (ISH), using the capacity of single nucleic acid chains to couple with complementary base pair sequences, have permitted the identification and localization of specific DNA or RNA sequences in morphologically intact cells or in chromosome spreads, and the development of molecular cloning methods provides a virtually unlimited range of nucleic acid probes to use in such analyses. The principles involved and the broad areas of application in haematological cytology and cytogenetics are described in a new section of Chapter 2, and referred to where relevant in later chapters.
目次
- History, general principles and procedures,and potential value of cytochemical methods in haematology
- Nucleoprotein cytochemistry and immunocytochemistry, flow cytometry, in situ hybridization techniques and applications in cytogenetics
- Other proteins, amino acids, and immunocytochemical methods for antigenic proteins
- Carbohydrates / Lipids and sudanophilia
- Iron and other inorganic elements
- Phosphatases
- Esterases
- Dehydrogenases
- Oxidases, peroxidases and catalases
- Other enzymes
- Cytochemical findings in cultured haemic cells
- Major cytochemical findings in non-neoplastic pathological haemopoiesis
- Major cytochemical findings in leukaemias, preluekaemic myelodysplasias and related states
- Major cytochemical and histochemical findings in lymphocyte subpopulations and in normal, reactive and lymphomatous lymph nodes. lymphocyte subpopulations and in normal, reactive and lvmphomatous lymph nodes.
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