Core techniques
著者
書誌事項
Core techniques
（The practical approach series, v. 148 . DNA cloning : a practical approach ; 1）
IRL Press, c1995
2nd ed
- : hbk
- : pbk
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注記
Includes bibliographical references and indexes
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199634767
内容説明
This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to date
account of each topic by established researchers.
These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.
目次
- 1. Techniques for transformation of E.coli
- 2. Lambda replacement vectors in the construction of genomic DNA libraries
- 3. Procedures for cDNA cloning
- 4. Making probes
- 5. Using cloned DNAs to analyse gene organization and expression
- 6. Choices of single-stranded vectors and their application for in vitro mutagenesis
- 7. PCR and its applications
- 8. DNA sequencing
- 巻冊次
-
: hbk ISBN 9780199634774
内容説明
Part of a four-volume set on DNA cloning, this particular book describes all of the necessary background and detailed laboratory methods for the core techniques used in every molecular biology laboratory.
目次
- Techniques for transformation of E.coli
- Lambda replacement vectors in the construction of genomic DNA libraries
- Procedures for cDNA cloning
- Making probes
- Using cloned DNAs to analyze gene organization and expression
- Choices of single-stranded vectors and their application for in vitro mutagenesis
- PCR and its applications
- DNA sequencing.
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