Core techniques

Author(s)

• Glover, David M.
• Hames, B. David

Bibliographic Information

Core techniques

edited by D.M. Glover and B.D. Hames

（The practical approach series, v. 148 . DNA cloning : a practical approach ; 1）

IRL Press, c1995

2nd ed

• : hbk
• : pbk

Note

Includes bibliographical references and indexes

Description and Table of Contents

Volume

: pbk ISBN 9780199634767

Description

This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to date account of each topic by established researchers. These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

Table of Contents

• 1. Techniques for transformation of E.coli
• 2. Lambda replacement vectors in the construction of genomic DNA libraries
• 3. Procedures for cDNA cloning
• 4. Making probes
• 5. Using cloned DNAs to analyse gene organization and expression
• 6. Choices of single-stranded vectors and their application for in vitro mutagenesis
• 7. PCR and its applications
• 8. DNA sequencing

Volume

: hbk ISBN 9780199634774

Description

Part of a four-volume set on DNA cloning, this particular book describes all of the necessary background and detailed laboratory methods for the core techniques used in every molecular biology laboratory.

Table of Contents

• Techniques for transformation of E.coli
• Lambda replacement vectors in the construction of genomic DNA libraries
• Procedures for cDNA cloning
• Making probes
• Using cloned DNAs to analyze gene organization and expression
• Choices of single-stranded vectors and their application for in vitro mutagenesis
• PCR and its applications
• DNA sequencing.

by "Nielsen BookData"

• NCID: BA25029811
• ISBN:
  • 0199634777
  • 0199634769
• LCCN: 94026740
• Country Code: uk
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Oxford ; Tokyo
• Pages/Volumes: xviii, 269 p.
• Size: 24 cm
• Classification:
  • LCC : QH442.2
  • DC20 : 574.87/3282
• Subject Headings:
  • LCSH : Molecular cloning -- Laboratory manuals
  • LCSH : Recombinant DNA -- Laboratory manuals
• Parent Bibliography ID:
  • BA00227768