DNA fingerprinting in plants and fungi

著者

書誌事項

DNA fingerprinting in plants and fungi

Kurt Weising ... [et al.]

CRC Press, c1995

  • : pbk

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注記

Includes bibliographical references (p. 245-286) and index

Other authors: Hilde Nybom, Kirsten Wolff, Wieland Meyer

内容説明・目次

内容説明

This exciting new book describes the use of DNA fingerprinting and its application in a wide area of plant and fungal research. It presents a thorough theoretical introduction to DNA fingerprinting, the practical aspects of extraction of DNA from the plant or fungus under study, and the statistical analysis of the data. An overview presents all species to which DNA fingerprinting is currently being applied and highlights many future technical developments.

目次

Genetic Variation at the DNA Level. A Short Survey of Molecular Markers. Minisatellites and Simple Sequences: The Molecular Basis of Classical DNA Fingerprinting. Detection of DNA Polymorphisms by PCR-Based Fingerprinting. Laboratory Equipment. Methodology. Safety Precautions. DNA Isolation and Purification. DNA Fingerprinting Based on Hybridization. PCR-Based DNA Fingerprinting. Evaluation of Fragment Patterns. Applications of DNA Fingerprinting in Plants and Fungi. Development of Methods and Initial Investigations. Wild Plant Species. Cultivated Plant Species. Inheritance of DNA Fingerprints, Linkage Analysis and Genome Mapping in Plants. Fungi: Human Pathogens, Plant Pathogens, Insect Pathogens, Non-Pathogenic Fungi, and Commercially Important Fungi. Conclusions. Comparison of Methods for Detecting Genetic Variation. Morphological Characters and Allozymes vs. DNA Markers. Different Kinds of Molecular Markers. Future Prospects. Improvement of Existing Methods. Extending the Spectrum of DNA Typing Strategies. References. Appendices. Index.

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