CiNii Books - Handbook of molecular and cellular methods in biology and medicine

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Table of Contents

Isolation and Purification of DNA Isolation and Purification of RNA Extraction and Purification of Proteins/Enzymes Preparation of Nucleic Acid Probes Electrophoresis, Blotting, and Hybridization cDNA Libraries Genomic DNA Libraries DNA Sequencing PCR Techniques and Applications DNA Fingerprinting DNA Mutagenesis In Vitro Inhibition of Gene Expression by Antisense DNA and RNA DNA Footprinting and Gel Retardation Assay Translation of mRNA(s) In Vitro and Analysis of Proteins by Gel Electrophoresis Gene Transfer and Expression in Animals Gene Transfer and Expression in Plants Plant Cell Culture Plant Tissue Culture Microscopy: LM, SEM, TEM, Confocal and Applications Bioseparation Techniques and Their Applications Preparation of Monoclonal and Polyclonal Antibodies against Specific Protein(s)

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Handbook of molecular and cellular methods in biology and medicine

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