Cryopreservation and freeze-drying protocols
著者
書誌事項
Cryopreservation and freeze-drying protocols
（Methods in molecular biology / John M. Walker, series editor, 38）
Humana Press, 1995
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book provides detailed protocols for all the latest methodologies used to assure the long-term biostorage of a diverse range of biological materials. Developed in expert laboratories, the protocols have been painstakingly perfected over the years to provide time-tested, step-by-step instructions that ensure robust and reproducible results. Each protocol deals with the preservation of an organelle, cell, or tissue type, and is accessible even to the nonspecialist because of its cookbook approach. Novel protocols can be readily developed with the help of the "hands-on" Notes sections. Cryopreservation and Freeze-Drying Protocols is an indispensable reference work for both the individual researcher, and all those who want to establish or improve biostorage systems in their laboratories. Its applications range from microbial culture collections, botanic gardens, and zoos to animal husbandry, aquaculture, medicine, human fertilization, and cell and molecular biology.
目次
Overview. Virus Cryopreservation and Storage. Freeze-Drying and Cryopreservation of Bacteria. Freeze-Drying Yeast. Cryopreservation of Yeast Cultures. Cryopreservation and Freeze-Drying of Fungi. Cryopreservation of Pathogenic and Nonpathogenic Free-living Amoebae. Long-Term Cryopreservation of Thylakoid Membranes. Cryopreservation of Algae. Cryopreservation of Plant Protoplasts. A Two-Step or Equilibrium Freezing Procedure for the Cryopreservation of Plant Cell Suspensions. Vitrification of Plant Cell Suspensions. Cryopreservation of Shoot-Tips and Meristems. Cryopreservation of Seeds. Cryopreservation of the Sperm of the Pacific Oyster Crassostrea gigas. Cryopreservation of Fish Spermatozoa. Cryopreservation of Avian Spermatozoa. Cryopreservation of Animal and Human Cell Lines. Cryopreservation of Semen from Domestic Livestock. Cryopreservation of Mammalian Embryos: Slow Cooling. Cryopreservation of Mammalian Embryos: Vitrification. Cryopreservation of Human Gametes. Cryopreservation of Human Red Blood Cells. Index.
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