Gene cloning : an introduction
著者
書誌事項
Gene cloning : an introduction
Chapman and Hall, 1995
3rd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
The third edition of "Gene Cloning" is a fully revised and updated version of this introductory text on the subject. It is longer and contains greater detail, yet is still aimed at undergraduates and at those who have little or no previous experience of cloning techniques. There are two additions to the contents. The first is a chapter on the polymerase chain reaction, a technique that has assumed great importance in molecular biology. The second is a final chapter on plant genetic engineering, reflecting the increasing research interest, and general concern, in promoting environmentally sensitive practices in agriculture. It is in a larger format than previous editions and is printed in two colours. It should be of interest to first and second year life sciences undergraduates, especially those reading biochemistry, molecular biology, biotechnology, cell biology and medicine.
目次
- The basic principles of gene cloning - why gene cloning is important
- vehicles - plasmids and bacteriophages
- purification of DNA from living cells
- manipulation of purified DNA
- introduction of DNA into living cells
- cloning vectors for E. coli
- cloning vectors for organisms other than E. coli
- the applications of cloning in gene analysis
- how to obtain a clone of a specific gene
- studying gene and genome structure
- studying gene expression
- the polymerase chain reaction
- gene cloning in research and biotechnology
- production of protein from cloned genes
- gene cloning in medicine
- gene cloning in agriculture
- what to read next.
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