Antibody engineering
著者
書誌事項
Antibody engineering
（Breakthroughs in molecular biology）
Oxford University Press, 1995
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book will help researchers to understand and develop designer antibodies. It provides a practical overview on how to use state-of-the-art technologies in antibody engineering, for the production of recombinant human or mouse monoclonal antibodies. Subjects such as antibody structure relevant to antibody engineering, plant and mammalian expression vectors and hosts, recombinatorial DNA libraries, PCR cloning of single cells, engineering of affinity and
biological effector functions, are thoroughly explored by some of the leading experts in each field.
目次
1: Introduction: Recombinant Antibodies: Future Trends
2: Human Monoclonal Antibodies from V-Gene Repertoiries Expressed on Bacteriophage
3: Synthetic Antibodies
4: Engineering the Antibody Combining Site by Codon-Based Mutagenesis in a Filamentous Phage Display System
5: Human Metaphoric Antibodies from a Phagemid Library
6: Strategies for Humanizing Antibodies
7: Single-Chain Fv Design and Production by Preparative Folding
8: Expressing Antibodies in Escherichia Coli
9: Vector and Approaches for the Eukaryotic Expression of Antibodies and Antibody Fusion Proteins
10: The Cloning of Hybridoma V Regions for their Ectopic Expression in Intracellular and Intercellular Immunisation
11: The Expression of 'Single Chain' Antibodies in Transgenic Plants
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