Cell growth and apoptosis : a practical approach

Author(s)

• Studzinski, George P.

Bibliographic Information

Cell growth and apoptosis : a practical approach

edited by George P. Studzinski

（The practical approach series, no. 159）

IRL Press, 1995

• : pbk.

Note

Includes bibliographical references and index

Description and Table of Contents

Volume

: pbk. ISBN 9780199635689

Description

This book offers a unique combination of succinctly expressed basic concepts of cell growth and cell death, particularly by apoptosis, and detailed instructions and protocols on how accurately to measure these processes. Experts in each line of these investigations describe a range of techniques which will enable weve an inexperienced reader to determine with precision such cellular events as the cell cycle traverse, chromosomal abnormalities detected at cell division, DNA damage that causes these abberrations, and cell death, or cell senescence. Establishment of the cell lines for studies is illustrated with reference to leukaemic cells, and optimization of cell proliferation by growth factors is also described. Together, these contributions form a useful laboratory manual for studies of growth abnormalities in human cell.

Table of Contents

• 1. Measuring parameters of growth
• 2. Cell cycle analysis by flow cytometry
• 3. Analysis of flow cytometric DNA histograms
• 4. Determination of the cell proliferative fraction in human tumors
• 5. Chromosome analysis in cell culture
• 6. Assessment of DNA damage in mammalian cells by DNA filter elution methodology
• 7. Morphological and biochemical criteria of apoptosis
• 8. Analysis of cell death by flow cytometry
• 9. Assays of cell growth and cytotoxicity
• 10. Cell synchronization as a basis for investigating control of proliferation in mammalian cells
• 11. Growth and activation of human leukaemic cells in vitro and their growth in the SCID mouse model
• 12. Senescence and immortalization of human cells
• 13. Analysis of the effects of purified growth factors on normal human fibroblasts

Volume

ISBN 9780199635696

Description

This text provides up-to-date procedures for studying the growth of cell populations. The practical instructions are accompanied by explanatory material which allows readers to choose which particular protocol is best for their purposes. The methods range from simple techniques, such as autoradiography and cell staining, to the more complex, such as flow cytometry.

Table of Contents

1: Measuring parameters of growth. 2: Cell cycle analysis by flow cytometry. 3: Analysis of flow cytometric DNA histograms. 4: Determination of the cell proliferative fraction in human tumors. 5: Chromosome analysis in cell culture. 6: Assessment of DNA damage in mammalian cells by DNA filter elution methodology. 7: Morphological and biochemical criteria of apoptosis. 8: Analysis of cell death by flow cytometry. 9: Assays of cell growth and cytotoxicity. 10: Cell synchronization as a basis for investigating control of proliferation in mammalian cells. 11: Growth and activation of human leukaemic cells in vitro and their growth in the SCID mouse model. 12: Senescence and immortalization of human cells. 13: Analysis of the effects of purified growth factors on normal human fibroblasts

by "Nielsen BookData"

• NCID: BA25851271
• ISBN:
  • 0199635692
  • 0199635684
• LCCN: 95003378
• Country Code: uk
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Oxford ; New York
• Pages/Volumes: xxvi, 269 p.
• Size: 24 cm
• Classification:
  • LCC : QH604.7
  • DC20 : 574.87/61
  • NDC7 : 491.311
• Subject Headings:
  • LCSH : Cells -- Growth -- Research -- Methodology
  • LCSH : Apoptosis -- Research -- Methodology
• Parent Bibliography ID:
  • BA00227768