Gene transfer to plants
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書誌事項
Gene transfer to plants
（Springer lab manual）
Springer-Verlag, c1995
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Includes bibliographical references
内容説明・目次
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ISBN 9783540584063
内容説明
Using the clearly structured protocols given in this manual, it will be easy to apply the latest techniques in plant biotechnology in order to create new plant varieties or varieties with altered and optimized characteristics. Direct gene transfer into plant cells or protoplasts by the use of microinjection, electroporation or biolistic systems or mediated by Agrobacteria is described in detail for various plant species, including relevant crops and cereals. Also included are protocols of the standard molecular techniques for the analysis of transgenic plants as well as a section on biosafety considerations and regulations.
- 巻冊次
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[pbk.] ISBN 9783642489679
内容説明
Gene Transfer to Plants Within a decade of the first recoveries of transgenic "model" plants, gene trans- fer is an established and routine technique in numerous laboratories around the world. It contributes to the rapid progress in basic and applied plant sciences in disciplines as diverse as biochemistry, physiology, developmental biology, breeding, food sciences, and biotechnology (Lindsey 1992; Willmitzer and Topfer 1992; Kishore and Somerville 1993; Nessler 1994). Following years of unsuccessful experiments with variations in feeding iso- lated DNA to plant tissues and organs, gene transfer became a reality soon after it was discovered that the soil bacterium Agrobacterium tumefaciens contained a plasmid, part of it being transferred to competent plant cells (see Hooykaas; Introduction I: Agrobacterium tumefaciens, a natural vector system). Host range limitations of Agrobacterium-mediated gene transfer prompted the search for alternative gene transfer systems, leading soon to the development of "direct gene transfer to protoplasts" (see Potrykus; Introduction III: Direct gene trans- fer to protoplasts).
Further limitations in both gene transfer systems led to the exploration of a great variety of further approaches such as pollen transforma- tion, pollen tube pathway, electrophoresis, microlaser, liposome-fusion and -injection, macroinjection, direct DNA application in numerous variations, etc. None of these approaches has, so far, been developed to a reproducible gene transfer technique and it is questionable whether they ever led to transformation (for a detailed assessment see Potrykus 1990).
目次
I Agrobacterium tumefaciens-Mediated Gene Transfer.- 1 Transformation of Arabidopsis thaliana Using Root Explants.- 2 Seed Transformation in Arabidopsis thaliana.- 3 In Planta Agrobacterium-Mediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum-Infiltration.- 4 Agrobacterium-Mediated Transformation of Potato (Solanum tuberosum).- 5 Agrobacterium-Mediated Transformation of Rapeseed (Brassica napus).- II Agrobacterium rhizogenes-Mediated Gene Transfer.- 6 Agrobacterium rhizogenes-Mediated Transformation: Transformed Roots to Transformed Plants.- III Direct Gene Transfer to Protoplasts.- 7 Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants.- 8 Polyethylene Glycol-Mediated Direct Gene Transfer to Indica Rice Protoplasts and Regeneration of Transgenic Plants.- 9 Plastid Transformation by Polyethylene Glycol Treatment of Protoplasts and Regeneration of Transplastomic Tobacco Plants.- 10 Electroporation-Mediated Gene Transfer to Protoplasts and Regeneration of Transgenic Plants.- 11 Electroporation Mediated Gene Transfer to Japonica Rice Protoplasts and Regeneration of Transgenic Plants.- 12 Regeneration of Transgenic Maize Plants from Embryogenic Protoplasts After Polyethylene Glycol-Mediated DNA Uptake.- 13 Gene Transfer to Protoplasts: Transient Gene Expression Analysis.- IV Biolistic Plant Transformation.- 14 Transient Gene Expression Studies with the Biolistic System.- 15 Maize Transformation by Microprojectile Bombardment of Immature Embryos.- 16 Biolistic Transformation of Microspore-Derived and Immature Zygotic Embryos and Regeneration of Fertile Transgenic Barley Plants.- 17 Transformation of Soybean (Glycine max) Through Electric Discharge Particle Acceleration.- 18 Ballistic Microtargeting of Visible Marker Genes to the Shoot Meristem of Wheat.- 19 Stable Transformation of Wheat by Microprojectile Bombardment.- 20 Biolistic-Based Analysis of Chloroplast Gene Expression.- V Microinjection and Fiber-Mediated Transformation.- 21 Microinjection into Tobacco Protoplasts and Regeneration of Transgenic Plants.- 22 Silicon Carbide Whisker-Mediated Transformation: Regeneration of Transgenic Maize Plants.- VI Tissue Electroporation.- 23 Transient Expression Following Electroporation into Immature Zygotic Embryos of Wheat.- 24 Transgenic Indica Rice Plants by Electroporation of Seed Embryo Cells.- VII Analysis of Transgenic Plants.- 25 Standard Molecular Techniques for the Analysis of Transgenic Plants.- 26 In Situ Hybridization on Plant Metaphase Chromosomes I. Farbos and A. Mouras.- 27 Localisation of RNA Transcripts in Plant Tissue by in Situ Hybridisation.- 28 In Situ Immunolocalisation of Transgene Products in Transgenic Plants.- VIII Establishment and Maintenance of Embryogenic Cultures.- 29 Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and Their Protoplasts in Forage Grasses.- IX Genetic Markers and Expression Signals.- 30 Expression Signals and Vectors.- 31 Selectable Marker and Reporter Genes.- X Guidelines and Biosafety Considerations.- 32 Release of Transgenic Plants: Regulations and Developments.- 33 Risk Assessment.
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