PCR : essential data
著者
書誌事項
PCR : essential data
（Essential data series / series editors, D. Rickwood, B.D. Hames）
J. Wiley, c1995
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注記
"Published in association with BIOS Scientific Publishers Limited" -- T.p
Bibliography: p. 180-208
Includes index
内容説明・目次
内容説明
This handy affordable lab reference allows quick access to fundamental techniques needed to undertake PCR. Topics covered include: background to PCR and its application; laboratory set--up; instruments; protocols; primers; detection and purification; methods for cloning PCR products; troubleshooting tips and more.
目次
Background to PCR and Its Application Areas (C. Newton). Setting Up a PCR Laboratory (C. Newton). Instruments (C. Newton). Nucleic Acid Substrates (C. Newton). Thermostable DNA Polymerases (C. Newton). Primers (C. Newton). The Synthesis of Chemically Labeled PCR Primers (T. Brown & D. Brown). Protocol Optimization and Reaction Specificity (S. Powell). Contamination Avoidance (C. Newton). Use of 5' Chemically Labeled Primers in PCR (J. Grzybowski, et al.). Detection of PCR Products (L. MacCallum). Purification of PCR Products (C. Newton). Cloning PCR Products (C. Newton). Miscellaneous Data (C. Newton). Troubleshooting (C. Newton). Manufacturers and Suppliers (C. Newton). References. Further Reading. Appendix. Index.
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