Cytokines : a practical approach
著者
書誌事項
Cytokines : a practical approach
（The practical approach series, 155）
IRL Press at Oxford University Press, c1995
2nd ed
- hard
- pbk.
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
pbk. ISBN 9780199635665
内容説明
Cytokines are proteins that control communication between cells and they are important in immunity, inflamation, tissue development, tissue repair, and cancer. This book is the new, revised edition of "Cytokines: A Practical Approach" published in 1991. It provides detailed, up-to-date practical information for the study of cytokines and cytokine-like activity and is also a guide to identifying and characterizing individual cytokines and provides a review of
cytokine biology. In addition, in providing a guide to some of the most useful techniques in cell and molecular biology, immunology, and biochemistry, it will be useful to workers in many different disciplines of biomedical research.
In this second edition, many important new techniques have been added including methods for developing cytokine transgenic mice, measuring soluble cytokine receptors, and assays for newly discovered cytokines (up to 1L-14). Each chapter has been updated in line with current experiments.
目次
- 1. Cloning and expression of cytokine genes
- 2. Southern blotting of cytokine genes
- 3. Northern analysis, ribonuclease protection and in situ analysis of cytokine messenger RNA
- 4. Polymerase chain reaction to quantitate cytokine mRNA
- 5. Comparative polymerase chain reaction
- 6. Receptor binding studies
- 7. Measurement of cytokine induction of PC-specific phospholipase C and sphingomyelinases
- 8. Two dimensional gel elctrophoresis for purification, sequencing and identification of cytokine-induced proteins in normal and malignant cells
- 9. Antiviral activity of cytokines
- 10. Assays for human B cell responses to cytokines
- 11. T cells and cytokines
- 12. The generation and quantitation of cell-mediated toxicity
- 13. Assays for chemotaxis
- 14. Cytokine regulation of endothelial cells
- 15. Assays for haematopoietic growth factors
- 16. Assays for macrophage activation by cytokines
- 17. Measurement of proliferative, cytolytic and cytostatic activity of cytokines
- 18. Production of cytokine transgenic and knockout mice
- 19. Development of antibodies to cytokines
- 20. Visualizing the production of cytokines and their receptors by single human cells
- 21. Quantitative biological assays for individual cytokines
- 22. RIA, IRMA and ELISA assays for cytokines and their soluble receptors
- 巻冊次
-
hard ISBN 9780199635672
内容説明
Cytokines are important mediators of immunity, inflammation, and cell growth and are relevant to many areas of biomedical research. This book, now in its second edition, provides detailed up-to-date practical information for the study of cytokines and their receptors, including guides to many of the most useful current techniques in cell and molecular biology, immunology, and biochemistry. In all it contains 184 protocols, providing a comprehensive resource for researchers in many disciplines of biomedical research. This book is intended for postgraduate and postdoctural researchers in immunology, infectious diseases, autommunity, oncology, and biomedical research in general.
目次
1: Cloning and expression of cytokine genes. 2: Southern blotting of cytokine genes. 3: Northern analysis, ribonuclease protection and in situ analysis of cytokine messenger RNA. 4: Polymerase chain reaction to quantitate cytokine mRNA. 5: Comparative polymerase chain reaction. 6: Receptor binding studies. 7: Measurement of cytokine induction of PC-specific phospholipase C and sphingomyelinases. 8: Two dimensional gel elctrophoresis for purification, sequencing and identification of cytokine-induced proteins in normal and malignant cells. 9: Antiviral activity of cytokines. 10: Assays for human B cell responses to cytokines. 11: T cells and cytokines. 12: The generation and quantitation of cell-mediated toxicity. 13: Assays for chemotaxis. 14: Cytokine regulation of endothelial cells. 15: Assays for haematopoietic growth factors. 16: Assays for macrophage activation by cytokines. 17: Measurement of proliferative, cytolytic and cytostatic activity of cytokines. 18: Production of cytokine transgenic and knockout mice. 19: Development of antibodies to cytokines. 20: Visualizing the production of cytokines and their receptors by single human cells. 21: Quantitative biological assays for individual cytokines. 22: RIA, IRMA and ELISA assays for cytokines and their soluble receptors
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