Agrobacterium protocols
著者
書誌事項
Agrobacterium protocols
（Methods in molecular biology / John M. Walker, series editor, 44）
Humana Press, c1995
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Agrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and physiology of transformed tissues and transgenic plants. Drawing on the work of leading scientists from laboratories around the world, Agrobacterium Protocols provides a wealth of techniques for introducing specific DNA sequences into target plant species and discusses the environmental implications of genetically engineered plants. Its detailed procedures will facilitate rapid transfer of advanced techniques to other laboratories and their exploitation in fundamental and applied plant biology.
目次
Growth and Storage of Agrobacterium. Simple Cultural Tests for Identification of Agrobacterium Biovars. Agrobacterium Virulence. Agrobacterium tumefaciens Chemotaxis Protocols. Effect of Acetosyringone on Growth and Oncogenic Potential of Agrobacterium tumefaciens. Binary Ti Plasmid Vectors. Leaf Disk Transformation. Agrobacterium-Mediated Transformation of Protoplasts from Oilseed Rape (Brassica napus L.). Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.). Peanut Transformation. Transformation of Soybean (Glycine max) via Agrobacterium tumefaciens and Analysis of Transformed Plants. Agrobacterium-Mediated Transformation of Potato Genotypes. Agrobacterium-Mediated Transformation of Soft Fruit Rubus Ribes and Fragaria. High-Frequency and Efficient Agrobacterium-Mediated Transformation of Arabidopsis thaliana Ecotypes C24 and Landsberg erecta Using Agrobacterium tumefaciens. Transformation Protocols for Broadleaved Trees. Agrobacterium-Mediated Antibiotic Resistance for Selection of Somatic Hybrids: The Genus Lycopersicon as a Model System. Histochemical GUS Analysis. Fluorometric GUS Analysis for Transformed Plant Material. The Detection of Neomycin Phosphotransferase Activity in Plant Extracts. The Plant Oncogenes rolA B and C from Agrobacterium rhizogenes: Effects on Morphology Development and Hormone Metabolism. Quantifying Polyamines in Agrobacterium rhizogenes Strains and in Ri Plasmid Transformed Cells. IAA Analysis in Transgenic Plants. Quantifying Phytohormones in Transformed Plants. Manipulating Photosynthesis in Transgenic Plants. Gene Activation by T-DNA Tagging. Assessing Cadmium Partitioning in Transgenic Plants. Overepression of Chloroplastic Cu/Zn Superoxide Dimutase in Plants. Agroinfection. T-DNA Transfer to Maize Plants. Use of Cosmid Libraries in Plant Transformations. Regulation of Agrobacterium Gene Manipulation. Transgenic Oilseed Rape: How to Assess the Risk of Outcrossing to Wild Relatives. Electroporation Protocols for Agrobacterium. Index.
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