Gene probes
著者
書誌事項
Gene probes
（The practical approach series, 161-162）
IRL Press at Oxford University Press, c1995
- 1 : hbk
- 1 : pbk
- 2 : hbk
- 2 : pbk
大学図書館所蔵 全22件
注記
Includes bibliographical references
収録内容
- 1. Preparation
- 2. Applications
内容説明・目次
- 巻冊次
-
1 : pbk ISBN 9780199634002
内容説明
The preparation of gene probes is a core application in molecular biology creating essential tools for genetic research. This volume together with its forthcoming companion volume, Gene Probes 2, provides a timely and important resource for all researchers in this field. Gene Probes 1 focuses on the wide range of techniques now available for the purification, synthesis, labelling, detection, and quantitation of probes, including
antibody probes. Gene Probes 2 will concentrate on describing techniques for specific research goals.
Contents List: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of oligodeoxynucleotide probes; Probe preparation by PCR; Synthesis of labelled riboprobes; Antibody probes
目次
- Introduction
- 1. Strategies for using gene probes
- 2. Preparation of nucleic acids for use as probes
- 3. DNA radiolabelling and detection
- 4. Non-radioactive labelling of DNA probes
- 5. Preparation of synthetic oligodeoxynucleotide probes
- 6. Probe preparation of PCR
- 7. Synthesis of labelled riboprobes
- 8. Antibody probes
- Appendix 1
- Appendix 2
- 巻冊次
-
1 : hbk ISBN 9780199634019
内容説明
The preparation of gene probes is a core application in molecular biology, creating essential tools for genetic research. A companion volume, Gene Probes 2 , covers applications of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in importance. This book is intended for this book will be of interest to researchers in a wide variety of fields e.g. molecular biology, molecular genetics, pathology, and developmental biology.
目次
Introduction. 1.: Strategies for using gene probes. 2.: Preparation of nucleic acids for use as probes. 3.: DNA radiolabelling and detection. 4.: Non-radioactive labelling of DNA probes. 5.: Preparation of synthetic oligodeoxynucleotide probes. 6.: Probe preparation of PCR. 7.: Synthesis of labelled riboprobes. 8.: Antibody probes. Appendix 1. Appendix 2
- 巻冊次
-
2 : pbk ISBN 9780199634149
内容説明
Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to analysis of human genetic mutations. A Companion volume, Genes Probes 1, covers preparation of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in
importance.
目次
- 1. Hybridization strategy
- 2. Screening recombinant DNA libraries by hybridization and amplification
- 3. Screening human recombinant libraries with nucleic acid probes
- 4. Antibody screening of bacterial expression systems
- 5. Isolation of cDNA clones by direct expression in mammalian cells
- 6. Genomic mapping and gene structure analysis
- 7. Analysis of gene expression by blotting techniques
- 8. In situ hybridization of nucleic acid probes to cellular RNA
- 9. Applications of chromosomal in situ hybridization
- 10. Analysis of gene expression analysis by PCR: a quantitative approach
- 11. Analysis of protein expression by immunocytochemistry
- 12. Gene targeting in mammalian cells
- 13. Directed mutagenesis and mutant analysis
- 14. Human gene mutational analysis
- Appendix 1
- Appendix 2
- 巻冊次
-
2 : hbk ISBN 9780199634156
内容説明
Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to the analysis of human genetic mutations. A companion volume covers the preparation of these probes. The volumes aim to contribute to a growing field of research.
目次
1.: Hybridization strategy. 2.: Screening recombinant DNA libraries by hybridization and amplification. 3: Screening human recombinant libraries with nucleic acid probes. 4.: Antibody screening of bacterial expression systems. 5.: Isolation of cDNA clones by direct expression in mammalian cells. 6.: Genomic mapping and gene structure analysis. 7.: Analysis of gene expression by blotting techniques. 8.: In situ hybridization of nucleic acid probes to cellular RNA. 9.: Applications of chromosomal in situ hybridization. 10.: Analysis of gene expression analysis by PCR: a quantitative approach. 11.: Analysis of protein expression by immunocytochemistry. 12.: Gene targeting in mammalian cells. 13.: Directed mutagenesis and mutant analysis. 14.: Human gene mutational analysis. Appendix 1. Appendix 2
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