Gene probes

Author(s)

• Hames, B. D.
• Higgins, S. J.

Bibliographic Information

Gene probes

edited by B. D. Hames and S. J. Higgins

（The practical approach series, 161-162）

IRL Press at Oxford University Press, c1995

• 1 : hbk
• 1 : pbk
• 2 : hbk
• 2 : pbk

Note

Includes bibliographical references

Contents of Works

• 1. Preparation
• 2. Applications

Description and Table of Contents

Volume

1 : pbk ISBN 9780199634002

Description

The preparation of gene probes is a core application in molecular biology creating essential tools for genetic research. This volume together with its forthcoming companion volume, Gene Probes 2, provides a timely and important resource for all researchers in this field. Gene Probes 1 focuses on the wide range of techniques now available for the purification, synthesis, labelling, detection, and quantitation of probes, including antibody probes. Gene Probes 2 will concentrate on describing techniques for specific research goals. Contents List: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of oligodeoxynucleotide probes; Probe preparation by PCR; Synthesis of labelled riboprobes; Antibody probes

Table of Contents

• Introduction
• 1. Strategies for using gene probes
• 2. Preparation of nucleic acids for use as probes
• 3. DNA radiolabelling and detection
• 4. Non-radioactive labelling of DNA probes
• 5. Preparation of synthetic oligodeoxynucleotide probes
• 6. Probe preparation of PCR
• 7. Synthesis of labelled riboprobes
• 8. Antibody probes
• Appendix 1
• Appendix 2

Volume

1 : hbk ISBN 9780199634019

Description

The preparation of gene probes is a core application in molecular biology, creating essential tools for genetic research. A companion volume, Gene Probes 2 , covers applications of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in importance. This book is intended for this book will be of interest to researchers in a wide variety of fields e.g. molecular biology, molecular genetics, pathology, and developmental biology.

Table of Contents

Introduction. 1.: Strategies for using gene probes. 2.: Preparation of nucleic acids for use as probes. 3.: DNA radiolabelling and detection. 4.: Non-radioactive labelling of DNA probes. 5.: Preparation of synthetic oligodeoxynucleotide probes. 6.: Probe preparation of PCR. 7.: Synthesis of labelled riboprobes. 8.: Antibody probes. Appendix 1. Appendix 2

Volume

2 : pbk ISBN 9780199634149

Description

Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to analysis of human genetic mutations. A Companion volume, Genes Probes 1, covers preparation of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in importance.

Table of Contents

• 1. Hybridization strategy
• 2. Screening recombinant DNA libraries by hybridization and amplification
• 3. Screening human recombinant libraries with nucleic acid probes
• 4. Antibody screening of bacterial expression systems
• 5. Isolation of cDNA clones by direct expression in mammalian cells
• 6. Genomic mapping and gene structure analysis
• 7. Analysis of gene expression by blotting techniques
• 8. In situ hybridization of nucleic acid probes to cellular RNA
• 9. Applications of chromosomal in situ hybridization
• 10. Analysis of gene expression analysis by PCR: a quantitative approach
• 11. Analysis of protein expression by immunocytochemistry
• 12. Gene targeting in mammalian cells
• 13. Directed mutagenesis and mutant analysis
• 14. Human gene mutational analysis
• Appendix 1
• Appendix 2

Volume

2 : hbk ISBN 9780199634156

Description

Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to the analysis of human genetic mutations. A companion volume covers the preparation of these probes. The volumes aim to contribute to a growing field of research.

Table of Contents

1.: Hybridization strategy. 2.: Screening recombinant DNA libraries by hybridization and amplification. 3: Screening human recombinant libraries with nucleic acid probes. 4.: Antibody screening of bacterial expression systems. 5.: Isolation of cDNA clones by direct expression in mammalian cells. 6.: Genomic mapping and gene structure analysis. 7.: Analysis of gene expression by blotting techniques. 8.: In situ hybridization of nucleic acid probes to cellular RNA. 9.: Applications of chromosomal in situ hybridization. 10.: Analysis of gene expression analysis by PCR: a quantitative approach. 11.: Analysis of protein expression by immunocytochemistry. 12.: Gene targeting in mammalian cells. 13.: Directed mutagenesis and mutant analysis. 14.: Human gene mutational analysis. Appendix 1. Appendix 2

by "Nielsen BookData"

Details

• NCID: BA26600404
• ISBN:
  • 0199634017
  • 0199634009
  • 0199634157
  • 0199634149
• LCCN: 95018740
• Country Code: uk
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Oxford ; New York
• Pages/Volumes: 2 vols
• Size: 24 cm
• Classification:
  • LCC : QP624.5.D73
  • DC20 : 574.87/322
• Subject Headings:
  • LCSH : DNA probes
• Parent Bibliography ID:
  • BA00227768